Time effect of chronic fluoride treatment in mice with induced steatosis

• Grant number: 13/25756-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2014
• End date: May 04, 2016
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Aline Dionizio Valle
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated scholarship(s): 15/10988-9 - Analysis of de novo lipogenesis by nuclear magnetic ressonance (NMR) in mice with fatty liver chronically treated with fluoride for 20 days, BE.EP.MS

Abstract

In a previous work by our group has noted that fluoride (F) when administered in conjunction with a calorie diet at different times may diminish the lipid droplets in liver and alter the lipid profile, indicating that this ion may lead to metabolic changes protective the objective of this study is to evaluate the effect of time of chronic administration of F in mice with hepatic steatosis induced by a high fat diet. 120 male Swiss mice with 28 days will be used. Initially the animals (n = 120) will be divided into 2 groups (n = 60 per group) will receive 30 days fat diet and drinking water without added F, to induce hepatic steatosis (Group I) or control diet and water drinking without adding F (group II). After this period, the group I will be divided into two groups (n = 30 per group) according to the type of drinking water given as follows: deionized drinking water (group A) , high-fat diet and drinking water with 50 ppm F (group B). The group II will be divided into two groups (n = 30 per group) according to the type of drinking water given as follows: deionized drinking (group C) and drinking water containing 50 ppm F Water (group D). In both cases , the feed will be maintained initially administered . In addition, each subgroup is divided into three subsubgrupos (n = 10 animals per subsubgrupo) in accordance with the time of treatment with water containing F or not , namely, 10 days (A1, B1, C1, D1) 20 days (A2, B2, C2 and D2) and 30 days (A3, B3, C3 and D3). After the experimental period, the animals will be anesthetized in 0.5 mL/kg body weight of Anasedan + 1.5 mL/kg of ketamine (Vetaset) for collection of liver and blood. F analysis will be made in plasma and liver tissue with the ion-specific electrode , after hexamethyldisiloxane -facilitated diffusion. Also the fixing of the liver to make the slides for histological analysis will be made. The remaining liver is separated for analysis of triglycerides. Of the plasma is used to analyze the lipid profile. After verification of normality and homoscedasticity of the data, the same shall be subjected to appropriate statistical analysis (p < 0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIONIZIO, ALINE; BARBOSA SILVA PEREIRA, HELOISA APARECIDA; ARAUJO, TAMARA TEODORO; SABINO-ARIAS, ISABELA TOMAZINI; FERNANDES, MILENI SILVA; OLIVEIRA, KARINA APARECIDA; RAYMUNDO, FABIELLE SALES; CESTARI, TANIA MARY; NOGUEIRA, FERNANDO NEVES; CARVALHO, RUI ALBUQUERQUE; et al. Effect of Duration of Exposure to Fluoride and Type of Diet on Lipid Parameters and De Novo Lipogenesis. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 190, n. 1, p. 157-171, JUL 2019. (15/10988-9, 13/25756-0)