Visible light during mycelial growth alters gene expression and improves stress tolerance in entomopathogenic fungi

• Grant number: 13/25964-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2014
• End date: April 30, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Drauzio Eduardo Naretto Rangel
• Grantee: Luciana Pereira Dias
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Associated research grant: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi, AP.JP

Abstract

Visible light influences primary and secondary metabolism, growth, and sexual and asexual development in many fungal species. However, little is known about the effects of light during mycelial growth on the stress tolerance of the developing fungal conidia. In this study, conidia of several entomopathogenic fungi will be produced under continuous visible light and dark, and the conidial tolerance will be evaluated under different stress conditions including solar UV radiation, osmotic stress, and 4-nitroquinoline-1-oxide (a genotoxic agent). The cellular mechanism in which light changes the stress tolerance will be studied based on differential gene expression. In addition, the light-sensing machinery in M. robertsii (ARSEF 2575) will be studied based on deletion of the white collar-1 genes. The coding for proteins for white collar genes are best understood in the WC-1 photoreceptor of Neurospora crassa. The genes contain a LOV (light, oxygen, or voltage) domain, similar to that present in fungal blue-light receptors. The results will provide insight about the effect of light on conidia in several fungi used for insect control. The fungi whose conidia are more stress resistant could be used in large-scale production.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, LUCIANA P.; ARAUJO, CLAUDINEIA A. S.; PUPIN, BRENO; FERREIRA, PAULO C.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.. The Xenon Test Chamber Q-SUN (R) for testing realistic tolerances of fungi exposed to simulated full spectrum solar radiation. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 592-601, JUN 2018. (13/25964-2, 12/15204-8, 13/50518-6, 10/06374-1)

ARAUJO, CLAUDINEIA A. S.; DIAS, LUCIANA P.; FERREIRA, PAULO C.; MITTMANN, JOSANE; PUPIN, BRENO; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.. Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 621-628, JUN 2018. (15/24305-0, 13/25964-2, 16/11386-5, 12/15204-8, 13/50518-6, 10/06374-1)

ARAUJO, CLAUDINEIA A. S.; FERREIRA, PAULO C.; PUPIN, BRENO; DIAS, LUCIANA P.; AVALOS, JAVIER; EDWARDS, JESSICA; HALLSWORTH, JOHN E.; RANGEL, DRAUZIO E. N.. Osmotolerance as a determinant of microbial ecology: A study of phylogenetically diverse fungi. FUNGAL BIOLOGY, v. 124, n. 5, p. 273-288, MAY 2020. (13/25964-2, 18/20571-6, 13/50518-6, 10/06374-1)

DIAS, LUCIANA P.; PEDRINI, NICOLAS; BRAGA, GILBERTO U. L.; FERREIRA, PAULO C.; PUPIN, BRENO; ARAUJO, CLAUDINEIA A. S.; CORROCHANO, LUIS M.; RANGEL, DRAUZIO E. N.. Outcome of blue, green, red, and white light on Metarhizium robertsii during mycelial growth on conidial stress tolerance and gene expression. FUNGAL BIOLOGY, v. 124, n. 5, p. 263-272, MAY 2020. (13/25964-2, 16/11386-5, 12/15204-8, 13/50518-6, 18/20571-6, 10/06374-1)

DIAS, LUCIANA P.; ARAUJO, CLAUDINEIA A. S.; PUPIN, BRENO; FERREIRA, PAULO C.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.. The Xenon Test Chamber Q-SUN (R) for testing realistic tolerances of fungi exposed to simulated full spectrum solar radiation. FUNGAL BIOLOGY, v. 122, n. 6, p. 10-pg., 2018-06-01. (10/06374-1, 13/25964-2, 12/15204-8, 13/50518-6)

DIAS, LUCIANA P.; SOUZA, ROBERTA K. F.; PUPIN, BRENO; RANGEL, DRAUZIO E. N.. Conidiation under illumination enhances conidial tolerance of insect-pathogenic fungi to environmental stresses. FUNGAL BIOLOGY, v. 125, n. 11, p. 891-904, NOV 2021. (10/06374-1, 13/25964-2, 13/50518-6)

ACHEAMPONG, MAVIS A.; PUPIN, BRENO; DIAS, LUCIANA P.; SANTOS, MAIARA P.; BERNARDES, LUANA O.; PINHEIRO, MICHELE S.; FERREIRA, PAULO C.; MIKAELYAN, ARAM; MEDINA, HUMBERTO R.; ALDER-RANGEL, ALENE; et al. Physiological stress tolerance responses of the dung decomposer fungus Mucor circinelloides. FUNGAL BIOLOGY, v. 129, n. 4, p. 8-pg., 2025-06-01. (13/50518-6, 13/25964-2, 10/06374-1)

ARAUJO, CLAUDINEIA A. S.; DIAS, LUCIANA P.; FERREIRA, PAULO C.; MITTMANN, JOSANE; PUPIN, BRENO; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.. Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide. FUNGAL BIOLOGY, v. 122, n. 6, p. 8-pg., 2018-06-01. (10/06374-1, 13/25964-2, 12/15204-8, 13/50518-6, 16/11386-5, 15/24305-0)

COSTA, TACYANA P. C.; RODRIGUES, ELIANE M.; DIAS, LUCIANA P.; PUPIN, BRENO; FERREIRA, PAULO C.; RANGEL, DRAUZIO E. N.. Different wavelengths of visible light influence the conidial production and tolerance to ultra-violet radiation of the plant pathogens Colletotrichum acutatum and Fusarium fujikuroi. European Journal of Plant Pathology, v. 159, n. 1, NOV 2020. (10/06374-1, 13/25964-2, 13/50518-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DIAS, Luciana Pereira. Visible light and oxygen limitation during mycelial growth of entomopathogenic fungi alter gene expression and conidia tolerance to stress conditions. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de Lorena (EEL/ASDI) Lorena.