Pegylated immunoliposomes containing paclitaxel: preparation, characterization, and antitumor activity

• Grant number: 14/03228-5
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: July 01, 2014
• End date: June 30, 2015
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Vananélia Pereira Nunes Geraldo
• Supervisor: Vladimir P. Torchilin
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Institution abroad: Northeastern University, United States
• Associated to the scholarship: 13/04246-4 - Immobilization of dapsone in biomimetic systems: self-assembled, Langmuir and Langmuir-Blodgett films, BP.PD

Abstract

The development of novel liposome formulations is crucial for enhanced drug delivery systems. In this Project, we shall prepare and characterize liposomes functionalized with polyethylene glycol (PEG) and the monoclonal antibody 2C5, which will be used as vehicles for the anti-cancer drug paclitaxel, widely used for treating breast and ovarian cancer. The major challenge is to incorporate paclitaxel in long-circulating immunoliposomes in order to increase the drug specificity and reduce its side effects. The latter include hypersensitivity reactions, nephrotoxicity and neurotoxicity. The drug delivery systems will be characterized by dynamic light scattering (DLS), zeta potential measurements, and transmission electron microscopy (TEM). To assess the cell interaction of the multifunctional liposomes, flow cytometry (FACS) analysis of HeLa and 4T1 cells incubated with liposomal formulations will be performed. It is hoped to understand the molecular-level interactions that may lead highly specific, effective drug delivery systems for cancer therapies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SRIRAMAN, SHRAVAN KUMAR; GERALDO, VANANELIA; LUTHER, ED; DEGTEREV, ALEXEI; TORCHILIN, VLADIMIR. Cytotoxicity of PEGylated liposomes co-loaded with novel pro-apoptotic drug NCL-240 and the MEK inhibitor cobimetinib against colon carcinoma in vitro. JOURNAL OF CONTROLLED RELEASE, v. 220, n. A, p. 160-168, DEC 28 2015. (14/03228-5)

SRIRAMAN, SHRAVAN KUMAR; GERALDO, VANANELIA; LUTHER, ED; DEGTEREV, ALEXEI; TORCHILIN, VLADIMIR. Cytotoxicity of PEGylated liposomes co-loaded with novel pro-apoptotic drug NCL-240 and the MEK inhibitor cobimetinib against colon carcinoma in vitro. JOURNAL OF CONTROLLED RELEASE, v. 220, p. 9-pg., 2015-12-28. (14/03228-5)