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Effect of jump squat training and detraining on mitochondrial gene expression of skeletal muscle of obese rats

Grant number: 13/27143-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2014
Effective date (End): December 31, 2016
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:Patricia Monteiro Seraphim
Grantee:Thais Batista Dutra
Home Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil

Abstract

The desire to leverage and endure physiological gains or ideal homeostatic state of the organism dates back to historical periods of humanity. Any type of stress to the organism can cause adaptation, such as increase of mitochondrial volume, with increased expression of protein PGC -1a, which can be changed by temperature, nutrient availability and training or detraining. With behavioral changes in dietary patterns, some chronic degenerative diseases are manifested in a systematic way, such as obesity, type 2 diabetes and insulin resistance, all involving inflammatory state. The physical training is a useful and cheap tool to improve glucose uptake in a state of insulin resistance presented with obesity. This is possible through of an activation of an insulin-independent pathway and that causes increased glucose transporter translocation to the plasma membrane involving the protein AMPK. The objective of this study is to evaluate the effect of vertical jumping training with weights and detraining on genes expression involved in glucose uptake and mitochondrial density, and improved anaerobic capacity of skeletal muscle of rats. The animals will be divided into 8 groups : E - Exercise , OE - Obese Exercise , S - Sedentary , OS - Obese Sedentary ; ED5 - Exercise Untrained five months-old; S5 - Sedentary five months-old ; OED5 - Obese Exercise Untrained five months-old; OS5 - Obese Sedentary five months-old . After the random selection, rats will be submitted to the intervention of intermittent training jumps in jump squat type with ballast weight in the back during 8 weeks and detraining for 4 weeks. Serum samples will be collected for lactic acid analysis every two weeks of training. After sacrifice the animals will be anesthetized with sodium pentobarbital anesthetic and gastrocnemius muscle will be extracted for the quantification of mRNA Prkaa2 gene (encodes AMPKa2 protein), Ucp3 and Ppargc1a (encodes Pgc1a protein) by RT -PCR. For statistical analysis as means of descriptive statistical, measures of central tendency (mean values) and measures of dispersion (standard deviation) will be used in the study. The analysis of variance (one-way ANOVA) will be used to analyze the existence of statistically significant differences between the averages. The Pearson product moment correlation is the statistical method adopted to attest to the existence of possible relationships between the variables analyzed. The Binary logistic regression (odds ratio) will be used to indicate the degree of extension of the associations between fatty supplementation and other variables studied. Before any abnormal distribution of the data statistical nonparametric equivalents of the above procedures will be used. Regardless of the type of data distribution p values less than 5% will be considered statistically significant. The software used for the statistical analysis of the data will be the SPSS version 15.0 (SPSS Inc, Chicago, IL).