Correlation of the granulocytic and monocytic cell profiles in the intestine of dogs naturally infected with Leishmania infantum

• Grant number: 13/13875-5
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): May 01, 2014
• Effective date (End): March 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Wilma Aparecida Starke Buzetti
• Grantee: Diogo Tiago da Silva
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil

Abstract

The Canine Visceral Leishmaniasis (CVL) is a parasitic infection caused by Leishmania (Leishmania) infantum, and is transmitted primarily by the bite of blood-sucking insects Lutzomyia longipalpis. Species of the genus Leishmania are intracellular parasites of macrophages in humans, dogs and a wide variety of wild animals, and if left untreated almost always result in the death of the mammal host. The objective of this study is to correlate the profile of granulocytic cells (neutrophils, eosinophils and mast cells), antigen-presenting cells - APC (dendritic cells and macrophages), T lymphocytes and Treg cells (by FOXP3 +) in naturally infected intestinal region of dogs by L. ( L. ) infantum with different degrees of parasitism in the intestinal tract and mesenteric lymph nodes and compare with the control group. The analysis of cell types will be based on three groups: positive dogs for LVC and the presence of the parasite in the intestine; positive for CVL and without the parasite in the intestine dogs, and non-infected dogs (control group). For this study samples of intestines of dogs euthanized by the city of Ilha Solteira or dead animals naturally (by accident or natural death) without parasitic coinfection Zoonosis Control Center (CCZ) will be collected. For detection of cell types (dendritic, macrophage, T cells, Treg cells and amastigotes of Leishmania), immunohistochemical techniques will be used, and for granulocytic cells, staining. The positivity of animals for LVC will be evaluated by performing serological methods (ELISA and IFAT) and molecular (PCR), and the parasites. Study the correlation between the different cell profiles, with the degree of parasitism, with injuries described and the clinical picture of the animal affected with CVL will also be undertaken.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIOGO TIAGO DA SILVA; MARIA LUANA ALVES; JÚLIO CESAR PEREIRA SPADA; ALINE CRISTINE DA SILVA; RITA DE CÁSSIA VIVEIROS DA SILVEIRA; TRÍCIA MARIA FERREIRA DE SOUSA OLIVEIRA; WILMA APARECIDA STARKE-BUZETTI. Linfócitos T e macrófagos nos tecidos intestinais de cães infectados com Leishmania infantum. Revista Brasileira de Parasitologia Veterinária, v. 26, n. 2, p. 159-170, 2017-06-00. (13/13875-5)

DA SILVA, DIOGO TIAGO; ALVES, MARIA LUANA; PEREIRA SPADA, JULIO CESAR; FRANCO LEONEL, JOAO AUGUSTO; BENASSI, JULIA CRISTINA; STARKE-BUZETTI, WILMA APARECIDA; FERREIRA, HELENA LAGE; KEID, LARA BORGES; SOARES, RODRIGO MARTINS; FERREIRA DE SOUSA OLIVEIRA, TRICIA MARIA. Leishmania infantum em órgãos reprodutivos de cães. Ciência Rural, v. 51, n. 10, 2021. (13/13875-5)

SILVA, DIOGO TIAGO; NEVES, MARIA FRANCISCA; GUAL PIMENTA DE QUEIROZ, NINA MARI; PEREIRA SPADA, JULIO CESAR; ALVES, MARIA LUANA; FLORO E SILVA, MARINA; DOURADO COELHO, WILLIAN MARINHO; PANOSSO, ALAN RODRIGO; FACONTI NORONHA JUNIOR, ANTONIO CARLOS; STARKE-BUZETTI, WILMA APARECIDA. Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum. Revista Brasileira de Parasitologia Veterinária, v. 25, n. 1, p. 24-36, JAN-MAR 2016. (13/13875-5)

DA SILVA, DIOGO TIAGO; ALVES, MARIA LUANA; PEREIRA SPADA, JULIO CESAR; VIVEIROS DA SILVEIRA, RITA DE CASSIA; FERREIRA DE SOUSA OLIVEIRA, TRICIA MARIA; STARKE-BUZETTI, WILMA APARECIDA. Neutrófilos, eosinófilos e mastócitos na parede intestinal de cães naturalmente infectados com Leishmania infantum. Revista Brasileira de Parasitologia Veterinária, v. 27, n. 4, p. 430-438, OCT-DEC 2018. (13/13875-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Diogo Tiago da. Estudo das células mononucleares e polimorfonucleares no intestino de cães naturalmente infectados por leishmania infantum. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Engenharia. Ilha Solteira Ilha Solteira.