|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||May 01, 2014|
|Effective date (End):||March 31, 2015|
|Field of knowledge:||Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine|
|Principal researcher:||Wilma Aparecida Starke Buzetti|
|Grantee:||Diogo Tiago da Silva|
|Home Institution:||Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil|
The Canine Visceral Leishmaniasis (CVL) is a parasitic infection caused by Leishmania (Leishmania) infantum, and is transmitted primarily by the bite of blood-sucking insects Lutzomyia longipalpis. Species of the genus Leishmania are intracellular parasites of macrophages in humans, dogs and a wide variety of wild animals, and if left untreated almost always result in the death of the mammal host. The objective of this study is to correlate the profile of granulocytic cells (neutrophils, eosinophils and mast cells), antigen-presenting cells - APC (dendritic cells and macrophages), T lymphocytes and Treg cells (by FOXP3 +) in naturally infected intestinal region of dogs by L. ( L. ) infantum with different degrees of parasitism in the intestinal tract and mesenteric lymph nodes and compare with the control group. The analysis of cell types will be based on three groups: positive dogs for LVC and the presence of the parasite in the intestine; positive for CVL and without the parasite in the intestine dogs, and non-infected dogs (control group). For this study samples of intestines of dogs euthanized by the city of Ilha Solteira or dead animals naturally (by accident or natural death) without parasitic coinfection Zoonosis Control Center (CCZ) will be collected. For detection of cell types (dendritic, macrophage, T cells, Treg cells and amastigotes of Leishmania), immunohistochemical techniques will be used, and for granulocytic cells, staining. The positivity of animals for LVC will be evaluated by performing serological methods (ELISA and IFAT) and molecular (PCR), and the parasites. Study the correlation between the different cell profiles, with the degree of parasitism, with injuries described and the clinical picture of the animal affected with CVL will also be undertaken.