Cytotoxicity of solvated and non-solvated with different concentrations of 2-hydroxietyl-metacrylate on pulp cells

• Grant number: 13/02928-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2014
• End date: December 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Adriano Fonseca de Lima
• Grantee: Lígia França Aires Zambelli
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

The aim of the present study will be evaluate the cytotoxicity of solvated and non-solvated adhesives with different amount of 2-hydroxyetil-metacrilate (HEMA), on dental pulp fibroblasts. The cells will be isolated from the dental pulp of sound human third molars. Model adhesive systems, solvated or not, will be manipulated with different concentration of HEMA (05, 10%, 20%, 30% and 40%). The fibroblasts 10 x 104 cells/well) will be seemed in sterile 6 wells dishes, and incubated. Cylindrical specimens (5mm diameter, 1 mm thick) will be prepared and maintained in contact with the culture medium for 6 h. After this, the obtained extracts (culture medium + products released from the adhesive system) will be applied on the cells and maintained in contact for 6 h. After this time, the extract will be removed and the cell metabolism will be analyzed using the metiltetrazolium (MTT assay). The obtained data will be tabulated and the statistical analysis will be performed.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MASSARO, HELDER; ZAMBELLI, LIGIA F. A.; DE BRITTO, AURILEIA A.; VIEIRA, RODOLFO P.; LIGEIRO-DE-OLIVEIRA, ANA P.; ANDIA, DENISE C.; OLIVEIRA, MARCELO T.; LIMA, ADRIANO F.. Solvent and HEMA Increase Adhesive Toxicity and Cytokine Release from Dental Pulp Cells. MATERIALS, v. 12, n. 17, SEP 2019. (13/02928-0, 12/15165-2)