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In vitro evaluation of alternative dentin conditioning agents based on ferric chloride and citric acid or nitric acid

Grant number: 18/09459-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2019
Effective date (End): May 31, 2021
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Marcelo Giannini
Grantee:Maicon Sebold
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):19/13613-7 - Evaluation of the physico-mechanical properties and proteolytic activity of resin-dentin bonds obtained with experimental dentin conditioning agents, BE.EP.DR


This study will evaluate the effects of dentin conditioning with 37% phosphoric acid, 10-3 solution (10% citric acid and 3% ferric chloride) or 1.4% nitric acid and post-etching dentin moisture on dentin bond strength, failure mode, ultramorphology, nanoleakage, mechanical properties and enzymatic activity of the dentin-adhesive interface, on the morphology of etched dentin, and on the transdentinal cytotoxicity caused by the conditioners. Two "universal" adhesive systems with different solvents (alcohol or acetone) will be tested on wet (positive controls) or dry (negative controls) demineralized dentin of human third molars. Adhesives will be applied to phosphoric acid-etched dentin (wet or dry), dry dentin previously treated with 10-3 solution or nitric acid (15 s), or in the self-etching mode. The bond strength test (n=8) and failure mode analysis will be performed at 24 hours and 18 months. Adhesive interface ultramorphology and nanoleakage will be analyzed by transmission electron microscopy (n=6). Bonding interface mechanical properties will be analyzed by nanoindentation (n=5), performing five equally spaced nanoindentations along the adhesive interface (load: 1000 µN). Enzymatic activity at the bonding interface will be determined by multifoton confocal microscopy (n=5), using fluorescein-conjugated gelatin. The morphology of etched dentin will be observed by scanning electron microscopy (n=5) using critical-point drying. Conditioners will by evaluated regarding cytotoxicity on odontoblast-like cells (MDPC-23) through dentin discs. Bond strength, nanoindentation, and cytotoxicity data will be analyzed by ANOVA and Tukey's test, while the remaining data will be analyzed descriptively. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SEBOLD, MAICON; ESTEVES LINS, RODRIGO BARROS; SAHADI, BEATRIZ OMETTO; SANTI, MARINA RODRIGUES; MARCONDES MARTINS, LUIS ROBERTO; GIANNINI, MARCELO. Microtensile Bond Strength, Bonding Interface Morphology, Adhesive Resin Infiltration, and Marginal Adaptation of Bulk-fill Composites Placed Using Different Adhesives. Journal of Adhesive Dentistry, v. 23, n. 5, p. 409-420, . (18/09459-0)
SEBOLD, MAICON; ANDRE, CAROLINA BOSSO; SAHADI, BEATRIZ OMETTO; BRESCHI, LORENZO; GIANNINI, MARCELO. Chronological history and current advancements of dental adhesive systems development: a narrative review. JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, v. 35, n. 18, p. 1941-1967, . (18/09459-0)

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