New approach to delivery beta: tricalcium phosphate nanoparticles for tooth remineralization

• Grant number: 22/00633-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 01, 2022
• End date: June 30, 2023
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Taís Scaramucci Forlin
• Grantee: Leonardo Custódio de Lima
• Supervisor: Sabrina Feitosa Sochacki
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Indiana University, United States
• Associated to the scholarship: 20/07986-2 - Synthesis of an experimental vehicle for the application of nanometric Beta-TCP particles functionalized with fluoride and stannous for the control of dentin surface loss and dentin hypersensitivity, BP.DR

Abstract

The aim of this study is to synthesize an experimental adhesive containing ²-TCP nanoparticles and characterize it in terms of biological, chemical and physical properties. The variation factor will be experimental adhesive, in 3 levels: 1. Positive Control: Commercial Adhesive (SBMP); 2. Experimental adhesive with ²-TCP nanoparticles and 3. Experimental adhesive without ²-TCP nanoparticles. To characterize the experimental material, 1. degree of conversion (% DC); 2. Knoop hardness (KHN); 3. Flexural strength (FS) and 4. Cytotoxicity (% cell viability) will be evaulated. Experimental dentin adhesive resins will be prepared by mixing Bis-GMA, TEGDMA, HEMA (50/25/25 wt.%), and photo-initiators and inhibitors. ²-TCP nanoparticles will be incorporated into the resin mixture at distinct concentrations, according to a pilot study. For the cytotoxicity and hardness tests, disc-shaped specimens (10 mm in diameter × 1 mm thick) will be prepared for each group (n = 3). For the flexural strength test, bar-shaped specimens (25 mm long × 2 mm wide × 2 mm thick) will be prepared for each group (n = 5) according to the ISO 4049. Data will be analyzed statistically using appropriate methods (± = 0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DENUCCI, G. C.; LIMA, L. C.; EKERT, G. J.; SILVA, F.; SCARAMUCCI, T.; SOCHACKI, S.; HARA, A. T.. Dental Adhesive Protection Against Development of Noncarious Cervical Lesions. Operative Dentistry, v. 50, n. 3, p. 9-pg., 2025-05-01. (22/00633-2)

LIMA, LEONARDO CUSTODIO; SILVA, FLAVIA RODRIGUES OLIVEIRA; VIANA, ITALLO EMIDIO LIRA; DENUCCI, GIOVANNA CORREA; MUMAW, CHRISTEN LEIGH; WALKER, CHANDLER; HARA, ANDERSON T.; SCARAMUCCI, TAIS; SOCHACKI, SABRINA FEITOSA. Novel resin-based material containing β-tricalcium phosphate nanoparticles for the reduction of dentin permeability. Journal of Dentistry, v. 141, p. 9-pg., 2023-12-30. (22/00633-2, 20/07986-2)