Disturbances of Von Willebrand factor and hepatic synthesis of hemosthatic factors induced by experimental Bothrops jararaca envenomation

• Grant number: 13/20239-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2014
• End date: April 12, 2018
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcelo Larami Santoro
• Grantee: Camila Martos Thomazini
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The activity of Bothrops jararaca venom is broad and complex on biologic systems of preys, showing pro- and anti-hemostatic features. Venom proteins may alter by various approaches the hemostatic systems. Several systemic manifestations are described during B. jararaca envenomation, and bleedings are the most conspicuous one. Systemic bleeding is multifactorial, and patients manifest consumption coagulopathy with specific particularities that have not been completely elucidated. Restoration of hemostatic disorder occurs progressively after administration of specific antivenom, which neutralizes circulating venom. However, stimulatory mechanisms for this recovery are still unknown. Information about the kinetics of hepatic synthesis of clotting factors may assist on the treatment of patients. On the other hand, thrombocytopenia and platelet disfunction, as well as the presence of mucous bleeding, which shares clinical similarity with symptons of von Willebrand disease, are also commonly observed in Viperidae envenomation. Thrombotic microangiopathy has been reported to following snakebites, and the occurrence of intravascular hemolysis has been associated with this disease. Von Willebrand factor (vWF) and its cleavage enzyme, ADAMTS13, could be closely related to this process, such as it occurs in cases of thrombotic thrombocytopenic purpura, where a reduction of ADAMTS13 activity and platelet microthrombi are found, associated with thrombocytopenia and microngiopathic hemolytic anemia. Therefore, we aimed in this study: (1) to analyze the occurrence of quantitative and qualitative disturbances in von Willebrand factor (vWF) and its cleavage enzyme, ADAMTS13, during experimental B. jararaca envenomation; (2) to investigate the synthesis of coagulation factors during envenomation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

THOMAZINI, CAMILA MARTOS; SACHETTO, ANA TERESA AZEVEDO; DE ALBUQUERQUE, CYNTHIA ZACCANINI; DE MOURA MATTARAIA, VANIA GOMES; DE OLIVEIRA, ANA KARINA; SERRANO, SOLANGE MARIA DE TOLEDO; LEBRUN, IVO; BARBARO, KATIA CRISTINA; SANTORO, MARCELO LARAMI. Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom. PLoS Neglected Tropical Diseases, v. 15, n. 9, SEP 2021. (13/07467-1, 18/26015-8, 13/20239-8, 19/07618-6, 13/25177-0)

THOMAZINI, CAMILA MARTOS; SILVA SOARES, ROSANGELA DE PAULA; FLORES DA ROCHA, TANIA RUBIA; AZEVEDO SACHETTO, ANA TERESA; SANTORO, MARCELO LARAMI. Optimization of von Willebrand factor multimer analysis in vertical mini-gel electrophoresis systems: A rapid procedure. THROMBOSIS RESEARCH, v. 175, p. 76-83, MAR 2019. (13/20239-8, 13/25177-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

THOMAZINI, Camila Martos. Involvement of von Willebrand factor in the plaquetopenia of experimental poisoning by the Bothrops jararaca snake: participation of botrocetin and venom metalloproteinases. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.