Search for structural templates for the engineering of catalytic functions

• Grant number: 14/03768-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2014
• End date: April 30, 2016
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Alessandro Silva Nascimento
• Grantee: Erick Giancarlo Suclupe Farro
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The existence of a structure-function relationship in proteins is well known. Proteins evolved to fold into stable structures optimized for their functional roles. However, what can be the minimal information necessary in order to create a functional role? Different approaches have been proposed to address this issue. One of these approaches involves the concept of sparse spatial motifs to define the functional role of enzymes. In this approach, the atomic coordinates of a few amino acids are used to infer the enzyme activity. In this project we ought to test whether the insertion of sparse spatial motifs of a simple active site is sufficient to give functional elements to a different protein. For this purpose, we will look for protein structures that might serve as templates for the insertion of the spatial motif of a polysaccharide monooxygenase (previously known as glycoside hydrolase 61, GH61) containing two histidines and one tyrosine coordinating a cupper atom. The search for a template will be done using the software SMatch, developed in our group. The evaluation of the gain of activity will be done in two steps: the ability for binding the metal and the monooxygenase activity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FARRO, ERICK GIANCARLO S.; LEITE, ANA ELISA T.; SILVA, ISABELA A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; POLIKARPOV, IGOR; NASCIMENTO, ALESSANDRO S.. GH43 endo-arabinanase from Bacillus licheniformis: Structure, activity and unexpected synergistic effect on cellulose enzymatic hydrolysis. International Journal of Biological Macromolecules, v. 117, p. 7-16, OCT 1 2018. (14/06565-2, 15/13684-0, 14/03768-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FARRO, Erick Giancarlo Suclupe. Structural and biochemical characterization of arabinanases from Bacillus licheniformis. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.