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DNA extraction methods for the study of rumen microbial population in cattle fed with glycerin associated with oil

Grant number: 13/23851-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2014
Effective date (End): February 28, 2017
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Telma Teresinha Berchielli
Grantee:Yury Tatiana Granja Salcedo
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):15/17966-0 - Use of molecular techniques for rumen microbial ecosystem studies, BE.EP.DR


This project aims to evaluate changes in rumen fermentation, intestinal flow of fatty acids, microbial protein synthesis, digestibility coefficient of association of oil and glycerin in diets for beef cattle. In experiment 1 will be held to standardize the techniques used to quantify the microbial community, which will evaluate methods of sampling and preparation of samples of rumen contents and extraction of metagenomic DNA for the purpose of adapting a methodology for using the qPCR technique in quantification of ruminal microbiota. In experiment 2 will be assessed the changes in microbial populations with the extraction method selected in Experiment 1 and biohydrogenation of unsaturated fatty acids, and rumen fermentation parameters of 4 diets : a diet without adding glycerin or oil, a diet with inclusion glycerin ; diet with addition of oil and glycerin combination with a diet -oil emulsion. Will be used eight Nellore steers with an average weight of 480 kg and an average age of 30 months, cannulated in the rumen and duodenum. The animals will be distributed in a completely randomized with two 4x4 Latin squares. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SAN VITO, ELIAS; GRANJA-SALCEDO, YURY TATIANA; LAGE, JOSIANE FONSECA; OLIVEIRA, ANDRE SOAREZ; GIONBELLI, MATEUS PIES; MESSANA, JULIANA DUARTE; DALLANTONIA, ERICK ESCOBAR; REIS, RICARDO ANDRADE; BERCHIELLI, TELMA TERESINHA. Crude glycerin as an alternative to corn as a supplement for beef cattle grazing in pasture during the dry season. SEMINA-CIENCIAS AGRARIAS, v. 39, n. 5, p. 2215-2232, . (13/23851-6, 11/00060-8, 11/06409-2)
JOSE NETO, ANTONIO; GRANJA-SALCEDO, YURY TATIANA; MESSANA, JULIANA DUARTE; MALHEIROS, EUCLIDES BRAGA; REIS, RICARDO ANDRADE; PIRES, ALEXANDRE VAZ; BERCHIELLI, TELMA TERESINHA. Soybean hulls as feed substitute of ground corn can increase the fiber digestibility and bacterial fibrolytic profile of grazing Nellore steers during the rainy season. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 6, 3, p. 3577-3594, . (13/23851-6, 11/00060-8, 12/08284-5, 14/09033-1)
GRANJA-SALCEDO, YURY TATIANA; MESSANA, JULIANA DUARTE; DE SOUZA, VINICIUS CARNEIRO; LINO DIAS, ANA VERONICA; KISHI, LUCIANO TAKESHI; REBELO, LUCAS ROCHA; BERCHIELLI, TELMA TERESINHA. Effects of partial replacement of maize in the diet with crude glycerin and/or soyabean oil on ruminal fermentation and microbial population in Nellore steers. BRITISH JOURNAL OF NUTRITION, v. 118, n. 9, p. 651-660, . (13/23851-6, 15/17966-0)
GRANJA-SALCEDO, YURY TATIANA; RAMIREZ-USCATEGUI, RICARDO ANDRES; GUILLERMO MACHADO, ELWI; MESSANA, JULIANA DUARTE; KISHI, LUCIANO TAKESHI; LINO DIAS, ANA VERONICA; BERCHIELLI, TELMA TERESINHA. Studies on bacterial community composition are affected by the time and storage method of the rumen content. PLoS One, v. 12, n. 4, . (13/23851-6, 15/17966-0)
GRANJA-SALCEDO, YURY TATIANA; DE SOUZA, VINICIUS CARNEIRO; LINO DIAS, ANA VERONICA; GOMEZ-INSUASTI, ARTURO SAMUEL; MESSANA, JULIANA DUARTE; BERCHIELLI, TELMA TERESINHA. Diet containing glycerine and soybean oil can reduce ruminal biohydrogenation in Nellore steers. ANIMAL FEED SCIENCE AND TECHNOLOGY, v. 225, p. 195-204, . (13/23851-6, 15/17966-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SALCEDO, Yury Tatiana Granja. Methods of ruminal sample storage for microbiological studies and ruminal metabolism of nelore steers fed with oil-and glycerine association. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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