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Degradation and in situ ruminal microbial dynamics of different sources of protein for beef cattle

Grant number: 17/02034-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2017
Effective date (End): August 31, 2020
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal researcher:Telma Teresinha Berchielli
Grantee:Yury Tatiana Granja Salcedo
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The objective of this project is to evaluate the ruminal microbial diversity involved in the ruminal degradation of soybean meal, cottonseed meal, DGGS and glutenose, and correlate it with ruminal degradation of DM, CP and protein fractions of each ingredient. In each experimental period, after 20 days of adaptation of the animals to the diet, 112 nylon bags in situ containing 5 g of each protein source will be placed in the rumen of cannulated steers fed with the respective protein source. Four nylon bags will be placed in the animals' rumen in reverse chronological order at 48, 24,16, 6, 4, 2 e 0.5h before being removed and eight non-incubated nylon bags filled with the different ingredients will be used as control (0 h). The relative degradation of the biomass during rumen incubation will be determined by MS analysis according to the AOAC methods and CP by multiplication by 6.25 of the nitrogen determined in the LECO FP-528 nitrogen analyzer. CP insoluble in neutral detergent, CP insoluble acid detergent, non-protein nitrogen and soluble protein content of protein sources will be estimated according to Licitra et al. (1996). The nylon bags recovered from the rumen for microbiological analyzes will be washed with PBS buffer solution and immediately frozen and storage in liquid nitrogen and transported to the laboratory for the extraction of metagenomic DNA. Sequencing of the V4/V5 region of the bacterial 16S rRNA gene will be the method used for the identification and quantification of adhered ruminal bacteria. The results will be analyzed considering a 4 x 4 Latin square design, with measures repeated over time.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SIMIONI, TIAGO A.; TORRECILHAS, JULIANA A.; MESSANA, JULIANA D.; GRANJA-SALCEDO, YURY T.; SAN VITO, ELIAS; LIMA, ANA R. C.; SANCHEZ, JOAO M. D.; REIS, RICARDO A.; BERCHIELLI, TELMA T. Influence of growing-phase supplementation strategies on intake and performance of different beef cattle genotypes in finishing phase on pasture or feedlot. LIVESTOCK SCIENCE, v. 251, SEP 2021. Web of Science Citations: 0.
GOMES CARVALHO ALVES, KENIA LARISSA; GRANJA-SALCEDO, YURY TATIANA; MESSANA, JULIANA DUARTE; DE SOUZA, VINICIUS CARNEIRO; GENEROSO GANGA, MARIA JULIA; DETOGNI COLOVATE, PAULO HENRIQUE; KISHI, LUCIANO TAKESHI; BERCHIELLI, TELMA TERESINHA. Rumen bacterial diversity in relation to nitrogen retention in beef cattle. Anaerobe, v. 67, FEB 2021. Web of Science Citations: 1.
GOMEZ-INSUASTI, ARTURO SAMUEL; GRANJA-SALCEDO, YURY TATIANA; CASTILLO VARGAS, JULIAN ANDRES; MESSANA, JULIANA DUARTE; DE OLIVEIRA SADER, ANA PAULA; BERCHIELLI, TELMA TERESINHA. In vitro gas production and fatty acids biohydrogenation of diets containing different unsaturated fatty acids sources plus crude glycerin. TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, v. 45, n. 5, p. 944-954, 2021. Web of Science Citations: 0.
GRANJA-SALCEDO, YURY TATIANA; FERNANDES, RODOLFO MACIEL; DE ARAUJO, RAFAEL CANONENCO; KISHI, LUCIANO TAKESHI; BERCHIELLI, TELMA TERESINHA; DE RESENDE, FLAVIO DUTRA; BERNDT, ALEXANDRE; SIQUEIRA, GUSTAVO REZENDE. Long-Term Encapsulated Nitrate Supplementation Modulates Rumen Microbial Diversity and Rumen Fermentation to Reduce Methane Emission in Grazing Steers. FRONTIERS IN MICROBIOLOGY, v. 10, MAR 29 2019. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.