Effects of phototherapy with combination of lasers and LEDs in experimental model of Duchenne Muscular Dystrophy

• Grant number: 14/05203-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2014
• Effective date (End): May 31, 2017
• Field of knowledge: Engineering - Biomedical Engineering
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ernesto Cesar Pinto Leal Junior
• Grantee: Gianna Móes Albuquerque Pontes
• Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

Duchenne muscular dystrophy is a lethal disease caused by absence of dystrophin affecting one in every 3500 males. The pre-clinical model widely used to study this disease is the mdx mice. These animals have complete absence of protein expression of dystrophin in their organism. Low-power phototherapy (with lasers and LEDs) has been widely used to promote tissue regeneration, decreasing inflammation and pain relief, and it has been showing also positive results in delaying skeletal muscle fatigue in animals and humans, improving biochemical markers related to skeletal muscle recovery when this therapy is applied previously to exercise. Phototherapy seems, therefore, to act as a protective therapy to muscular tissue. With these factors in mind, this project aims to analyze gene and protein expression of different inflammatory and muscular markers, as well as analyze morphology and muscular function of mdx mice. For such, will be used different experimental groups that will be treated with phototherapy for 14 weeks. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALBUQUERQUE-PONTES, GIANNA MOES; CASALECHI, HELIODORA LEAO; TOMAZONI, SHAIANE SILVA; SERRA, ANDREY JORGE; BACELAR FERREIRA, CHEILA DE SOUSA; DE OLIVEIRA BRITO, RODRIGO BARBOSA; DE MELO, BRUNNO LEMES; VANIN, ADRIANE AVER; DAMASCENO SOARES MONTEIRO, KADMA KARENINA; DELLE, HUMBERTO; FRIGO, LUCIO; MARCOS, RODRIGO LABAT; CAMILLO DE CARVALHO, PAULO DE TARSO; PINTO LEAL-JUNIOR, ERNESTO CESAR. Photobiomodulation therapy protects skeletal muscle and improves muscular function of mdx mice in a dose-dependent manner through modulation of dystrophin. Lasers in Medical Science, v. 33, n. 4, p. 755-764, MAY 2018. Web of Science Citations: 4.