Association of antibiotics and antimicrobial photodynamic therapy for control of Acinetobacter baumannii

• Grant number: 14/03937-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2014
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Juliana Campos Junqueira
• Grantee: Naiara Pires Ramanzini
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Due to the rapid expansion of micro -organisms resistant to antibiotics and the limited development of new antimicrobial agents, infections by Gram - negative bacteria are becoming a challenge for health professionals and a threat to international public health. The aim of this study is to evaluate the synergistic effect of conventional antibiotics associated with antimicrobial photodynamic therapy (PDT) in the control strains of Acinetobacter baumannii. To perform this study, clinical strains of A. baumannii from clinical laboratories in São José dos Campos / SP will be obtained . All clinical strains will be subjected to tests to determine the minimal inhibitory concentration antibiotic imipenem and meropenem following the CLSI standards. Sensitive and antibiotic-resistant strains will be evaluated for in vitro susceptibility to antimicrobial photodynamic therapy. Also, will be tested the effects of conventional antibiotics, PDT and combined antibiotics and PDT in experimental infections in G. mellonella induced by clinical isolates of A. baumannii resistant to antibiotic therapy. The results of therapy for experimental infection will be evaluated by the survival curve of the larvae of G. mellonella. Data from in vitro tests will be submitted to ANOVA and Tukey's test. The data on the survival of G. mellonella curve will be analyzed by using the log -rank method. In all tests, it is assumed significance level of 5 % .

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELLO, MIRIAN MARCOLAN; DE BARROS, PATRICIA PIMENTEL; BERNARDES, RENATA DE CASSIA; ALVES, SILVIO RUBENS; RAMANZINI, NAIARA PIRES; ALVES FIGUEIREDO-GODOI, LIVIA MARA; CHIPOLETTI PRADO, ANA CAROLINA; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS. Antimicrobial photodynamic therapy against clinical isolates of carbapenem-susceptible and carbapenem-resistant Acinetobacter baumannii. Lasers in Medical Science, v. 34, n. 9, p. 1755-1761, DEC 2019. (14/03937-6, 12/15250-0)