Effect of phenolic compounds derived from pretreated biomass on cellulase and xylanase activities

• Grant number: 14/09157-2
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: July 01, 2014
• End date: December 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Michele Michelin
• Supervisor: Michael R. Ladisch
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Purdue University, United States
• Associated to the scholarship: 12/09889-8 - Development of Autohydrolysis Process of the Straw and Sugarcane Bagasse for the Production in Bioreactors of enzymes that degrade Biomass, BP.PD

Abstract

The exhaustive use and negative impact of fossil fuels on the environment lead to the search of renewable materials for the production of fuel alternatives. One of the possibilities is the production of bioethanol from lignocellulosic biomass; several steps are involved in this process, which must be optimized. The biomass pretreatment is one of the most critical steps since no optimum pretreatment was found till now. Besides, biomass pretreatment has the detrimental effect of release a wide range of compounds, such as phenolics, which are inhibitory to cellulolytic/hemicellulolytic enzymes, reducing their activities. Autohydrolysis pretreatment has been pointed as a good option for the biomass pretreatment since it is not considered as severe as the ones using chemical reagents. The aim of the present project (BEPE) is to determine and quantify the phenolic compounds that are released during the autohydrolysis pretreatment and analyze the inhibitory effect of these compounds during the enzymatic hydrolysis. The autohydrolysis process of straw and sugar cane bagasse and the production of cellulases and xylanases enzymes are part of the Project FAPESP 12/09889-8, being that the inhibitors analysis (BEPE) will be important to conclude this task of the current project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MICHELIN, MICHELE; XIMENES, EDUARDO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES; LADISCH, MICHAEL R.. Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities. Bioresource Technology, v. 199, p. 275-278, JAN 2016. (14/09157-2)