Synthesis of mesoporous silica nanoparticles functionalized with monoclonal antibodies: an alternative to Hodgkin (HL) and anaplastic large cell CD30+ (ALCL - CD30+) lymphomas treatment

• Grant number: 13/22429-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2014
• End date: May 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Mateus Borba Cardoso
• Grantee: Jessica Fernanda Affonso de Oliveira
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated scholarship(s): 15/21918-1 - Improvement of cell targeting through protein corona-resistant functionalization on doxorubicin and curcumin loaded mesoporous silica nanoparticles, BE.EP.DR

Abstract

The project here described aims to synthesize mesoporous silica nanoparticles to encapsulate doxorubicin for further functionalization with monoclonal antibodies for treating Hodgkin and anaplastic large cell CD-30+ lymphomas. As monoclonal antibodies exhibit highly specific binding sites, the aim of this work is to obtain a more effective chemotherapy for this type of lymphoma. In addition, the work has focused on: (1) analyze the specificity of the synthesized front patients and healthy cells and (2) to investigate the controlled release of the drug, reducing its toxicity. The structures formed will be characterized by ultraviolet visible spectroscopy (UV-Vis), small angle X-ray scattering (SAXS), transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermo-gravimetric analysis (TGA). Nitrogen adsorption/desorption isotherms will be used in determining the size and organization of the pores. The evolution of the nanoparticles functionalization will be investigated by Fourier transform infrared spectroscopy (FT-IR) and zeta potential. Biological activity experiments will be conducted with Hodgkin and anaplastic large cell CD-30+ lymphoma cells, which have the specific antigens that are recognized by antibodies present on the surface of the nanoparticles synthesized. The counting and analysis of cells will be performed with the Perkin Elmer Operetta and flow cytometer, both at National Biosciences Laboratory (LNBio). (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AFFONSO DE OLIVEIRA, JESSICA FERNANDA; DA SILVA, RAQUEL FRENEDOSO; SOUSA RIBEIRO, IRIS RENATA; SAITO, ANGELA; MELO-HANCHUK, TALITA DINIZ; DIAS, MARILIA MEIRA; KOBARG, JORG; CARDOSO, MATEUS BORBA. Selective Targeting of Lymphoma Cells by Monoclonal Antibody Grafted onto Zwitterionic-Functionalized Nanoparticles. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION, v. 37, n. 3, FEB 2020. (14/22322-2, 15/25406-5, 13/22429-9, 17/21318-0)

AFFONSO DE OLIVEIRA, JESSICA FERNANDA; CAPELETTI, LARISSA BRENTANO; CARDOSO, MATEUS BORBA. Are antibiotic-functionalized nanoparticles a promising tool in antimicrobial therapies?. Nanomedicine, v. 12, n. 23, p. 4-pg., 2017-12-01. (17/01167-7, 15/25406-5, 14/22322-2, 13/22429-9)

SHIMIZU, FLAVIO M.; PASQUALETI, ANIELLI M.; TODAO, FAGNER R.; DE OLIVEIRA, JESSICA F. A.; VIEIRA, LUIS C. S.; GONCALVES, SUELY P. C.; DA SILVA, GABRIELA H.; CARDOSO, MATEUS B.; GOBBI, ANGELO L.; MARTINEZ, DIEGO S. T.; et al. Monitoring the Surface Chemistry of Functionalized Nanomaterials with a Microfluidic Electronic Tongue. ACS SENSORS, v. 3, n. 3, p. 716-726, MAR 2018. (13/14262-7, 15/25406-5, 13/22429-9, 12/15543-7, 14/24126-6)

AFFONSO DE OLIVEIRA, JESSICA FERNANDA; SCHEFFER, FRANCINE RAMOS; LANDIS, RYAN F.; NETO, ERICO TEIXEIRA; ROTELLO, VINCENT M.; CARDOSO, MATEUS BORBA. Dual Functionalization of Nanoparticles for Generating Corona-Free and Noncytotoxic Silica Nanoparticles. ACS APPLIED MATERIALS & INTERFACES, v. 10, n. 49, p. 41917-41923, DEC 12 2018. (15/25406-5, 14/22322-2, 13/22429-9, 15/21918-1)

MELO FERREIRA, DANIELLE CRISTHINA; GIORDANO, GABRIELA FURLAN; DOS SANTOS PENTEADO SOARES, CAIO CESAR; AFONSO DE OLIVEIRA, JESSICA FERNANDA; MENDES, RENATA KELLY; PIAZZETTA, MARIA HELENA; GOBBI, ANGELO LUIZ; CARDOSO, MATEUS BORBA. Optical paper-based sensor for ascorbic acid quantification using silver nanoparticles. Talanta, v. 141, p. 188-194, AUG 15 2015. (11/21954-7, 13/22429-9)

CAPELETTI, LARISSA BRENTANO; AFFONSO DE OLIVEIRA, JESSICA FERNANDO; DIAS LOIOLA, LIVIA MESQUITA; GALDINO, FLAVIA ELISA; DA SILVA SANTOS, DENYS EWERTON; SOARES, THEREZA AMELIA; FREITAS, RAUL DE OLIVEIRA; CARDOSO, MATEUS BORBA. Gram-Negative Bacteria Targeting Mediated by Carbohydrate-Carbohydrate Interactions Induced by Surface-Modified Nanoparticles. ADVANCED FUNCTIONAL MATERIALS, v. 29, n. 48, NOV 2019. (17/01167-7, 13/22429-9, 15/25406-5, 18/09555-9, 16/21598-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Jessica Fernanda Affonso de. Synthesis of mesoporous silica nanoparticles functionalized with monoclonal antibodies: an alternative for Hodgkin (HL) and Anaplastic Large Cell CD30+ (ALCL-CD30+) lymphomas treatment. 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.