Physiological, transcriptional and metabolites analysis of different developmental stages of sugarcane leaf +1

• Grant number: 14/03562-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2014
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcelo Menossi Teixeira
• Grantee: Denis Bassi
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Sugarcane is one of the most important crops in the world and Brazil is its principal producer. Bioethanol importance has increased investments on the acquisition of sugarcane varieties with higher sucrose content. It is known that when sugarcane is cultivated under high CO2 regime it responds with increased photosynthetic rate, plant height, biomass production, water use efficiency, industrial productivity and induction of genes related to photosynthetic system, thus, there is a genetic potential to increase sugarcane productivity. Although the leaf plays a key role in the sugar accumulation, physiological and transcriptional evaluation of the different developmental stages present along leaf blade has not been yet reported for sugarcane. The objective of this project is evaluate physiological, transcriptional and metabolites behavior of different segments from leaf +1 from sugarcane through (a) measurements of photosynthetic and fluorescence of chlorophyll a parameters; (b) expression of genes related to sink/source transition in order to evaluate tissue maturity along leaf blade; (c) evaluate sugar composition in each leaf segment e correlate them with photosynthetic and expression of sink/source genes; (d) evaluate N content in each leaf segment e correlate them with photosynthetic and expression of sink/source genes; (e) evaluate transcriptional profile using the RNA-seq technique from contrasting leaf segments along the day to identify those genes related to high photosynthetic rate assemble; (f) Measuring metabolite levels using metabolomic techniques and then to compare them with data from transcriptomics. Our results will allow the identification of new biotechnological targets that could be used to produce sugarcane varieties with higher agronomic performance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BASSI, DENIS; MENOSSI, MARCELO; MATTIELLO, LUCIA. Nitrogen supply influences photosynthesis establishment along the sugarcane leaf. SCIENTIFIC REPORTS, v. 8, FEB 2 2018. (14/03562-2, 12/23345-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BASSI, Denis. Análise fisiológica, transcricional e do perfil metabólico de diferentes estágios de desenvolvimento da folha +1 de cana de açúcar sob diferentes concentrações de nitrogênio (N). 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.