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The Leishmania-host relationship from the ‘omics’ perspective

Grant number: 18/23512-0
Support type:Research Projects - Thematic Grants
Duration: August 01, 2019 - July 31, 2024
Field of knowledge:Biological Sciences - Parasitology - Protozoology of Parasites
Principal researcher:Lucile Maria Floeter-Winter
Grantee:Lucile Maria Floeter-Winter
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Pesquisadores principais:
Monica Levy Andersen
Assoc. researchers: Angeles López-Gonzálvez ; Audun Helge Nerland ; Coral Barbas ; Eva Gluenz ; Jane Tomimori ; Juliana Ide Aoki ; Karl Erik Müller ; Maria Fernanda Laranjeira da Silva ; Maricruz Mamani Huanca ; Ricardo Andrade Zampieri ; Sandra Marcia Muxel
Associated scholarship(s):21/04422-3 - Metabolic impact on the regulation of the L-arginine transporter in the polyamines pathway of Leishmania amazonensis, BP.PD
20/03186-1 - Sleep fragmentation influence in the Th1/Th2 response to L. amazonensis infection of C57BL/6 mice, BP.IC

Abstract

Leishmania is the protozoan responsible for leishmaniasis, a neglected disease that in Brazil causes about 100,000 new cases per year. In its life cycle, the parasite is found in the midgut of an insect or inside the phagolysosome in the macrophage of the mammalian host. The different stages of parasite life cycle influence the regulation of gene expression, which occurs at post-transcriptional level. Applying genomic, transcriptomic and metabolomic approaches, we will continue, in four subprojects, to study the thematic established by our group, determining and/or establishing new correlations between arginine metabolism and polyamine production in the parasite-host interaction. Thus, we intend to characterize parasites resistant to pentamidine and duramycin, target drugs in polyamine pathways (subproject A). To determine how the infection changes the miRNA profile of human or mice macrophages correlating with transcripts and metabolites profiles (subproject B). To use CRISPR/Cas9 to obtain null knockouts parasites of genes involved in arginine metabolism, confirming physiological roles and the location of their products (subproject C). To correlate sleep fragmentation and consequent alteration of the mounting of immune response against Leishmania infection in C57BL/6 mice, elucidating if there are changes in the profile of transcripts and miRNAs that influence the immune response and the course of infection (subproject D). The challenge of the four sub-projects is to correlate the different aspects of the parasite-host interaction by "omic" analysis, raising points that can be explore the understanding of biology and physiology, and for the design of new strategies to combat the parasite. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MAMANI-HUANCA, MARICRUZ; MUXEL, SANDRA MARCIA; ACUNA, STEPHANIE MAIA; FLOETER-WINTER, LUCILE MARIA; BARBAS, CORAL; LOPEZ-GONZALVEZ, ANGELES. Metabolomic Reprogramming of C57BL/6-Macrophages during Early Infection with L. amazonensis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 13 JUL 2021. Web of Science Citations: 0.
SOUZA, MARINA DE ASSIS; RAMOS-SANCHEZ, EDUARDO MILTON; MUXEL, SANDRA MARCIA; LAGOS, DIMITRIS; REIS, LUIZA CAMPOS; PEREIRA, VALERIA REGO ALVES; BRITO, MARIA EDILEUZA FELINTO; ZAMPIERI, RICARDO ANDRADE; KAYE, PAUL MARTIN; FLOETER-WINTER, LUCILE MARIA; GOTO, HIRO. miR-548d-3p Alters Parasite Growth and Inflammation in Leishmania (Viannia) braziliensis Infection. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUN 10 2021. Web of Science Citations: 0.
HONG, AHYUN; ZAMPIERI, RICARDO ANDRADE; SHAW, JEFFREY JON; FLOETER-WINTER, LUCILE MARIA; LARANJEIRA-SILVA, MARIA FERNANDA. One Health Approach to Leishmaniases: Understanding the Disease Dynamics through Diagnostic Tools. PATHOGENS, v. 9, n. 10 OCT 2020. Web of Science Citations: 0.
ACUNA, STEPHANIE MAIA; FLOETER-WINTER, LUCILE MARIA; MUXEL, SANDRA MARCIA. MicroRNAs: Biological Regulators in Pathogen-Host Interactions. CELLS, v. 9, n. 1 JAN 2020. Web of Science Citations: 0.
MARCIA MUXEL, SANDRA; MAMANI-HUANCA, MARICRUZ; AOKI, JULIANA IDE; ZAMPIERI, RICARDO ANDRADE; FLOETER-WINTER, LUCILE MARIA; LOPEZ-GONZALVEZ, ANGELES; BARBAS, CORAL. Metabolomic Profile of BALB/c Macrophages Infected with Leishmania amazonensis: Deciphering L-Arginine Metabolism. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 24 DEC 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.