| Grant number: | 21/04422-3 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | June 01, 2021 |
| Effective date (End): | May 31, 2022 |
| Field of knowledge: | Biological Sciences - Parasitology - Protozoology of Parasites |
| Principal researcher: | Lucile Maria Floeter-Winter |
| Grantee: | Juliana Ide Aoki |
| Home Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Associated research grant: | 18/23512-0 - The Leishmania-host relationship from the ‘omics’ perspective, AP.TEM |
Abstract Leishmania is auxotrophic for amino acids and nutrients and has aspecific transport for L-arginine mediated by amino acid porter-3 (AAP3) inpromastigote and amastigote forms. This transporter is encoded in two copies in the parasite's genome, one of which is regulated by the presence of the amino acid itself, externally or internally, in addition to pH, temperature andparasite arginase enzyme activity. The PD proposal has as its central objective tocharacterize the physiological role and cell location of the L-arginine transporter AAP3 through the application of CRISPR / Cas9 gene editing technology. (AU) | |