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Metabolic impact on the regulation of the L-arginine transporter in the polyamines pathway of Leishmania amazonensis

Grant number: 21/04422-3
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2021
Effective date (End): May 31, 2022
Field of knowledge:Biological Sciences - Parasitology - Protozoology of Parasites
Principal researcher:Lucile Maria Floeter-Winter
Grantee:Juliana Ide Aoki
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:18/23512-0 - The Leishmania-host relationship from the ‘omics’ perspective, AP.TEM

Abstract

Leishmania is auxotrophic for amino acids and nutrients and has aspecific transport for L-arginine mediated by amino acid porter-3 (AAP3) inpromastigote and amastigote forms. This transporter is encoded in two copies in the parasite's genome, one of which is regulated by the presence of the amino acid itself, externally or internally, in addition to pH, temperature andparasite arginase enzyme activity. The PD proposal has as its central objective tocharacterize the physiological role and cell location of the L-arginine transporter AAP3 through the application of CRISPR / Cas9 gene editing technology. (AU)