Antifungal activity and genotoxicity of atmospheric pressure nonthermal plasma jet

• Grant number: 14/02354-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2014
• End date: June 30, 2016
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Cristiane Yumi Koga Ito
• Grantee: Aline Chiodi Borges
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Fungal diseases represent a great challenge to the medical and dental areas, due to the increasing prevalence, few therapeutic options and antifungal resistance. Cold- plasma jet is a gaseous mixture containing charged particles, free radicals and radiation. Its potential application in infectious disease was reported, however there is still a lack of a systematic study on the antifungal effect and its mechanism of action. Moreover, studies on its genotoxic potential were not detected. The general aim of this project is to evaluate the antifungal effect of helium plasma jet on Candida albicans and Trichophyton rubrum and its genotoxic potential. The antifungal effect on planctonic cells and biofilms will be determined. Effects on fungal cells and on virulence factors will be evaluated, searching for evidences of the mechanisms of action. For C. albicans, assays for analysis of cell wall and cell membrane integrity, ergosterol quantification, morphogenesis, exoenzymes production, adherence to epithelial cells and in vivo effect in the treatment of oral candidosis in murine model will be performed. For T. rubrum, membrane and cell wall integrity, biomass production, micelial radial growth, cell morphology, keratinase activity and effects in an ex vivo nail infection model will be evaluated. The genotoxic potential will be evaluated in vitro on cultures of keratinocytes and CHO cells by using comet test. Data will be statistically analyzed, first by testing the normality of the sample distribution and then, by specific adequate analysis (±=5%). (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TEODORO, GUILHERME RODRIGUES; LACERDA GONTIJO, ALINE VIDAL; BORGES, ALINE CHIODI; TANAKA, MARCIA HIROMI; GOUVEA LIMA, GABRIELA DE MORAIS; SALVADOR, MARCOS JOSE; KOGA-ITO, CRISTIANE YUMI. Gallicacid/hydroxypropyl-beta-cyclodextrin complex: Improving solubility for application on in vitro/in vivo Candida albicans biofilms. PLoS One, v. 12, n. 7, JUL 11 2017. (12/16805-5, 14/13469-0, 14/02354-7, 14/09666-4, 15/03470-3, 13/00037-1)

BORGES, ALINE CHIODI; GOUVEA LIMA, GABRIELA DE MORAIS; CASTALDELLI NISHIME, THALITA MAYUMI; LACERDA GONTIJO, ALINE VIDAL; KOSTOV, KONSTANTIN GEORGIEV; KOGA-ITO, CRISTIANE YUMI. Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study. PLoS One, v. 13, n. 6, JUN 27 2018. (16/07196-6, 14/02354-7, 15/21989-6)

BORGES, ALINE CHIODI; CASTALDELLI NISHIME, THALITA MAYUMI; KOSTOV, KONSTANTIN GEORGIEV; GOUVEA LIMA, GABRIELA DE MORAIS; LACERDA GONTIJO, ALINE VIDAL; MARTINS MARCHESOTTI DE CARVALHO, JULIANA NOBREGA; HONDA, ROBERTO YZUMI; KOGA-ITO, CRISTIANE YUMI. Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits. CLINICAL PLASMA MEDICINE, v. 7-8, p. 9-15, DEC 2017. (15/03470-3, 16/07196-6, 14/02354-7, 15/21989-6)

NISHIME, T. M. C.; BORGES, A. C.; KOGA-ITO, C. Y.; MACHIDA, M.; HEIN, L. R. O.; KOSTOV, K. G.. Non-thermal atmospheric pressure plasma jet applied to inactivation of different microorganisms. SURFACE & COATINGS TECHNOLOGY, v. 312, p. 19-24, FEB 25 2017. (14/02354-7)

BORGES, ALINE CHIODI; CASTALDELLI NISHIME, THALITA MAYUMI; ROVETTA, SABRINA DE MOURA; GOUVEA LIMA, GABRIELA DE MORAIS; KOSTOV, KONSTANTIN GEORGIEV; THIM, GILMAR PATROCINIO; CANUTO DE MENEZES, BEATRIZ ROSSI; BARROS MACHADO, JOAO PAULO; KOGA-ITO, CRISTIANE YUMI. Cold Atmospheric Pressure Plasma Jet Reduces Trichophyton rubrum Adherence and Infection Capacity. Mycopathologia, v. 184, n. 5, p. 585-595, OCT 2019. (14/02354-7, 15/21989-6, 16/07196-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BORGES, Aline Chiodi. Antifungal activity and citotoxicity of atmospheric pressure nonthermal plasma jet. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.