Toxicogenetics evaluation of Disperse Violet 93 (textile dye)in Swiss mice

• Grant number: 14/08856-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2014
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Carla Carolina Munari
• Grantee: Mário Otávio Botasso Nasciutti
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Azo dyes, the largest class of synthetic dyes, are widely used in several industries such as pharmaceutical, food, electronics and textile mainly. In textile branch, it is known that a percentage of these dyes are lost during the coloring process, which contributes to the huge quantity of such compounds in industrial wastewater. Moreover, these compounds are not completely eliminated by the conventional water treatment; unlike, this process can produce chlorinated aromatic compounds even more hazardous. Herein, in order to assess the risks of the presence of these compounds in the environmental, we purpose evaluate the in vivo genotoxicity of azo dye Disperse Violet 93 found in textile effluents and scarcely investigated. Liver, kidney and peripheral blood cells of mice will be analyzed by comet assay and the bone marrow by micronucleus test. We expected that the results of this project may contribute to better understanding the acute effect of Disperse Violet 93 and also generate public policies for the use and discarding of dyes.