Contribution of catecholaminergic neurons of the rostral ventrolateral medulla in the respiratory control

• Grant number: 14/07698-6
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2014
• Effective date (End): February 28, 2017
• Field of knowledge: Biological Sciences - Physiology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Thiago dos Santos Moreira
• Grantee: Milene Rodrigues Malheiros Lima
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The catecholaminergic (TH) neurons reside in the rostral half of the ventrolateral medulla (RVLM). These neurons use the glutamate as a neurotransmitter, and beyond catecholamine, synthetize other neuropeptides. C1 neurons, located in the RVLM, are distributed in the viscerotropic organization, and, in the most part, are intensely activated by hypoglycemia, infection or inflammation, hypoxia, nociception, and cardiovascular alterations. These neurons control the autonomic nervous system by projections to sympathetic and parasympathetic pre ganglionic neurons, and also to hypothalamic-pituitary axis. Moreover, recent evidences indicated that the C1 neurons could also regulate the breathing activity. Therefore, considering the several functions attributed to catecolaminergic C1 neurons, as well as their anatomical connection, we believe that C1 region could also be recruited during chemoreceptors activation, promoting respiratory adjustments. In the present project, we propose to perform selective stimulation and inhibition of the C1 neurons by using new technologies that allow the selective control of this region of the brain. The new technology use viral vector that, in association with optogenetic stimulation, induces selective infection and manipulation of the C1 neurons. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MALHEIROS-LIMA, MILENE R.; TOTOLA, LEONARDO T.; LANA, MARLOUS V. G.; STRAUSS, BRYAN E.; TAKAKURA, ANA C.; MOREIRA, THIAGO S. Breathing responses produced by optogenetic stimulation of adrenergic C1 neurons are dependent on the connection with preBotzinger complex in rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, v. 470, n. 11, p. 1659-1672, NOV 2018. Web of Science Citations: 3.

MALHEIROS-LIMA, MILENE R.; TOTOLA, LEONARDO T.; TAKAKURA, ANA C.; MOREIRA, THIAGO S. Impaired chemosensory control of breathing after depletion of bulbospinal catecholaminergic neurons in rats. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, v. 470, n. 2, p. 277-293, FEB 2018. Web of Science Citations: 2.

MALHEIROS-LIMA, MILENE R.; TAKAKURA, ANA C.; MOREIRA, THIAGO S. DEPLETION OF ROSTRAL VENTROLATERAL MEDULLARY CATECHOLAMINERGIC NEURONS IMPAIRS THE HYPDXIC VENTILATORY RESPONSE IN CONSCIOUS RATS. Neuroscience, v. 351, p. 1-14, MAY 20 2017. Web of Science Citations: 10.

HAWKINS, VIRGINIA E.; TAKAKURA, ANA C.; TRINH, ASHLEY; MALHEIROS-LIMA, MILENE R.; CLEARY, COLIN M.; WENKER, IAN C.; DUBREUIL, TODD; RODRIGUEZ, ELLIOT M.; NELSON, MARK T.; MOREIRA, THIAGO S.; MULKEY, DANIEL K. Purinergic regulation of vascular tone in the retrotrapezoid nucleus is specialized to support the drive to breathe. eLIFE, v. 6, APR 7 2017. Web of Science Citations: 16.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LIMA, Milene Rodrigues Malheiros. Catecholaminergic neurons of the brainstem contributes to respiratory adjusts induced by hypoxia and hypercapnia.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas São Paulo.