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EVALUATION, IN VITRO OF THE APPLICATION OF 17% EDTA AND ND:YAG LASER ON THE ADHESION OF PERIODONTAL LIGAMENT FIBROBLASTS ON THE ROOT SURFACE OF AVULSED TEETH

Grant number: 14/09245-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2014
Effective date (End): June 30, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Samira Esteves Afonso Camargo
Grantee:Laura Maria Alvarez Nosch
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The success of delayed reimplantation of avulsed teeth is limited due to the greatpotential of external root resorption occurs. Despite being a reality in the dental office,there isn't a specific treatment for this type of trauma. In an attempt to restore thearchitecture and function of the periodontal ligament, and to avoid ankylosis , avoidingor modulating the replacement resorption, the root surface of avulsed teeth can betreated with various substances including EDTA (ethylenediaminetetraacetic acid) 17%,fluoride at high concentrations, among others, always seeking a lower chance ofinflammatory resorption and replacement occurs. Thus, the aim of this study is toevaluate the effectiveness of the treatment of discs from the vestibular surface of rootsof bovine teeth with nd: yag laser associated with hydroxyapatite nanoparticles via cellviability and qualitative evaluation of images by microscopy tests. Seventy six dentindiscs will be cut with 4.5 mm in diameter after the scrape of the labial surface of theroot surface of bovine teeth with periodontal curette. The discs will be legalized,cleaned and autoclaved. The specimens will be treated with the recommendedsubstances and placed in 96 wells where cells of primary cultures of human periodontalligament fibroblasts will be in contact with the discs for 48 hours. Survival and cellviability on the surface of discs will be assessed by XTT assay. The scanning electronmicroscopy will be used to verify the adherence of fibroblasts to the surface of the discs.Data will be analyzed statistically by Anova and Tukey test (p < 0.05).