Calcium and phosphorous quantification on dental enamel submitted to bleaching agents containing high and low hydrogen peroxide concentrations

• Grant number: 14/08035-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2014
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Roberta Tarkany Basting Höfling
• Grantee: Ana Victoria Dourado Pinto
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

The aim of this in vitro study was to evaluate the calcium and phosphorous content in human dental enamel before, during and after bleaching treatments with hydrogen peroxide (HP). Forty eight third human molars will be divided into six groups (n = 8) to receive the application of "bleaching agents containing high concentration of HP" - Whiteness HP Maxx/ FGM (35% HP); Whiteness Blue HP / FGM (35% HP + 2% calcium gluconate); Pola Office +/ SDI (37.5% HP + 5% potassium nitrate); Opalescence Boost/ Ultradent (38% HP + 1.1% fluoride and 3% potassium nitrate) - and "bleaching agents containing low concentration of HP" - Pola Day/ SDI (9.5% HP); White Class 10%/ FGM (10% HP + potassium nitrate + calcium + fluoride). These agents will be applied to dental enamel surfaces according to the manufacturer's instructions. To determine the amount of calcium and phosphorous in enamel, samples form enamel surface will be obtained by using the microbiopsy enamel technique at different times (before, during and after bleaching treatment). The calcium and phosphorus amount will be obtained in micrograms per milliliter (g/ ml) by using total reflection of X-rays methodology (TXRF). The data will be tabulated into spreadsheets in Microsoft Office Excel 2012 for the statistical analysis and ANOVA will be applied if there is homogeneity of variance followed by Tukey test. Otherwise, non-parametric tests will be used.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, A. V. D.; BRIDI, E. C.; AMARAL, F. L. B.; FRANCA, F. M. G.; TURSSI, C. P.; PEREZ, C. A.; MARTINEZ, E. F.; FLORIO, F. M.; BASTING, R. T.. Enamel Mineral Content Changes After Bleaching With High and Low Hydrogen Peroxide Concentrations: Colorimetric Spectrophotometry and Total Reflection X-ray Fluorescence Analyses. Operative Dentistry, v. 42, n. 3, p. 308-318, MAY-JUN 2017. (14/08035-0, 14/08188-1)