Comparative proteomic analysis of liver Nelore cattle males of high and low feed efficiency

• Grant number: 14/04937-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2014
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Joanir Pereira Eler
• Grantee: Leydiana Duarte Fonseca
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

With this project aims to determine possible proteins or peptides present in the liver of Nelore cattle that may be associated with the phenotype of feed efficiency (FE). To this end, liver samples collected in confinement experiment conducted previously which was characterized Nelore males at high and low feed efficiency using residual intake and body weight gain indicator. Liver samples from 20 animals are used, with 10 animals classified as high FE and 10 animals as low FE, a total of 100 animals were collected during slaughter. The samples will be processed for the extraction and quantification of protein content. Later be separated by two-dimensional gel electrophoresis method, according to their isoelectric point and molecular weight. After separation, the gels are stained and scanned for visualization of spots. Upon detection of protein spots analyzes comparison will be performed. The spots that are classified as differentially expressed in the statistical analysis will go through the process of digestion in trypsin for identification by mass spectrometry after consulting the complete proteome of Bos taurus in stock UniProtKB/Swiss-Prot database. From the analyzes protein markers that are associated with FE may be detected. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FONSECA, LEYDIANA D.; ELER, JOANIR P.; PEREIRA, MIKAELE A.; ROSA, ALESSANDRA F.; ALEXANDRE, PAMELA A.; MONCAU, CRISTINA T.; SALVATO, FERNANDA; ROSA-FERNANDES, LIVIA; PALMISANO, GIUSEPPE; FERRAZ, JOSE B. S.; et al. Liver proteomics unravel the metabolic pathways related to Feed Efficiency in beef cattle. SCIENTIFIC REPORTS, v. 9, MAR 29 2019. (14/04937-0, 14/12492-8, 14/07566-2, 14/02493-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FONSECA, Leydiana Duarte. Proteomic characterization of the liver of Nellore cattle divergent for feed efficiency. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.