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Proteins characterization µ-calpain, m-calpain and calpastatin and their relation with the quality characteristics of Nelore (Bos indicus) selected for production meat.

Grant number: 11/14850-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): June 30, 2013
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal Investigator:Pedro de Magalhães Padilha
Grantee:Welder Angelo Baldassini
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:09/16118-5 - Genomic tools to genetic improvement of direct economic important traits in Nelore cattle, AP.TEM


This project aims to deepen the study of structural and functional characteristics of calpain and calpastatin proteins in Nelore (Bos indicus) from production selected herd, and their relation to the meat quality, specifically tenderness. The calcium-dependent proteolytic system called calpain-calpastatin is considered as a limiting factor in the myofibrillar proteins (protein turnover) synthesis and degradation. Thus, knowledge about this enzyme system allow it's manipulation, which can result in changes on myofibrillar proteins growth and degradation rate. For the experimental analysis about 2,000 Nelore (Bos indicus) will be used, feedlot finished with age less than two years, with available information about performance and pedigree. From the amount of 2000 slaughtered animals, 40 Nelore, divided into two experimental treatments, will be used for this test: (1) animals with the highest shear force values (n = 20) and (2) animals with the lowest shear force values (n = 20), in order to get two quite contrasting groups on the meat tenderness. Between all samples, one will be used for the shear force test (SF), another one for LM area, one for backfat thickness and finally one for marbling score. The remaining samples will be used for total lipid analysis, meat instrumental color and myofibrillar fragmentation index (MFI). Proteomics will be used to visualize differences in expression of these proteins related to environmental conditions and processing. Electrophoretic separations will be performed in two-dimensional gels (2D) electrophoresis system using a 2D PAGE. The mapping of calcium in protein spots will be done in the line of X-ray fluorescence with synchrotron radiation (SR-XRF). The protein spots will be identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BALDASSINI, WELDER ANGELO; BRAGA, CAMILA PEREIRA; LOYOLA CHARDULO, LUIS ARTUR; II VASCONCELOS SILVA, JOSINEUDSON AUGUSTO; MALHEIROS, JESSICA MORAES; DE ALBUQUERQUE, LUCIA GALVAO; FERNANDES, TALITA TANAKA; PADILHA, PEDRO DE MAGALHAES. Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus). Food Chemistry, v. 169, p. 65-72, . (09/16118-5, 11/14850-0, 10/51332-5)
BALDASSINI, W. A.; CHARDULO, L. A. L.; SILVA, J. A. V.; MALHEIROS, J. M.; DIAS, V. A. D.; ESPIGOLAN, R.; BALDI, F. S.; ALBUQUERQUE, L. G.; FERNANDES, T. T.; PADILHA, P. M.. Meat quality traits of Nellore bulls according to different degrees of backfat thickness: a multivariate approach. ANIMAL PRODUCTION SCIENCE, v. 57, n. 2, p. 363-370, . (09/16118-5, 11/14850-0, 10/51332-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BALDASSINI, Welder Angelo. Abordagem bioanalítica e físico-química da qualidade de carne em bovinos Nelore (Bos indicus) selecionados para produção. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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