An metalloproteomics and expression approach of the structural, proteolytic and tissue skeletal muscle regulatory proteins in post-mortem and the relationship with growth and meat tenderness in Nellore animals (Bos indicus).

• Grant number: 15/13021-1
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2015 - March 31, 2018
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Luis Artur Loyola Chardulo
• Grantee: Luis Artur Loyola Chardulo
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Henrique Nunes de Oliveira ; Maeli Dal Pai ; Pedro de Magalhães Padilha ; Rogério Abdallah Curi

Abstract

This project aims to conduct a more specifies approach on the characterization, expression and quantification of important proteins related with the growth of the skeletal muscle tissue and meat tenderness in of Nellore animals. For that will be used data and samples from 90 male animals Nellore whole aged approximately 27 months undergoing growth management and finishing in feedlot for 95 days. During the feedlot were taken measurements of weight and calculated the live weight gains in the periods. The animals were slaughtered at average live weight of 550 kg, when then were colleted samples for analysis of gene and protein expression. The carcasses were then cooled for 24 hours when they were collected samples of the Longissimus thoracis muscle to realization of the analyzes of production and of meat quality as: rib eye area, backfat thickness, instrumental color of the meat, shear force, cooking losses and determination of myofibrillar fragmentation index and intramuscular lipid content of the meat. The animal performance characteristic, carcass and meat quality will be related to the analytical studies of proteins. For this will be adopted specific bioanalytical methods as the separation of proteins by two-dimensional electrophoresis (2D-PAGE) with emphasis in the HSP family proteins and the calpain complex (calpain-I, calpain-II and calpastatin). There will also be performed the identify the calcium ions in the protein spots by X-ray fluorescence (XRF-SR) and characterization of proteins by mass spectrometry (ESI-MS). The gene expression analysis will be made (RT-PCR) of the myofibrillar protein myosin heavy chain (MyHC) and related postmortem proteolysis in as calpain-I, calpain-II and calpastatin. Assays quantitation of proteins by Western blot will also be performed for isoforms MyHC (I, IIa, IIb and IIx) for the calpain complex. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MALHEIROS, J. M.; ENRIQUEZ-VALENCIA, C. E.; BRAGA, C. P.; VIEIRA, J. C. S.; VIEIRA, D. S.; PEREIRA, G. L.; CURI, R. A.; MACHADO NETO, O. R.; OLIVEIRA, H. N.; PADILHA, P. M.; et al. Application of proteomic to investigate the different degrees of meat tenderness in Nellore breed. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (15/13021-1)

MALHEIROS, JESSICA MORAES; BRAGA, CAMILA PEREIRA; GROVE, RYAN ALBERT; RIBEIRO, FELIPE AZEVEDO; CALKINS, CHRIS RICHARD; ADAMEC, JIRI; LOYOLA CHARDULO, LUIS ARTUR. Influence of oxidative damage to proteins on meat tenderness using a proteomics approach. MEAT SCIENCE, v. 148, p. 64-71, FEB 2019. (15/13021-1)

MALHEIROS, JESSICA MORAES; ELENA ENRIQUEZ-VALENCIA, CRUZ; DA SILVA DURAN, BRUNO OLIVEIRA; DE PAULA, TASSIANA GUTIERREZ; CURI, ROGERIO ABDALLAH; DE VASCONCELOS SILVA, JOSINEUDSON AUGUSTO I. I.; DAL-PAI-SILVA, MAELI; DE OLIVEIRA, HENRIQUE NUNES; LOYOLA CHARDULO, LUIS ARTUR. Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. MEAT SCIENCE, v. 138, p. 49-52, APR 2018. (15/13021-1)

CHARDULO, L. A. L.; BALDASSINI, W. A.; CURI, R. A.; PEREIRA, G. L.; MACHADO NETO, O. R.; DAL-PAI, M.; VECHETTI-JUNIOR, I. J.; MALHEIROS, J. M.; ENRIQUEZ-VALENCIA, C. E.. Gene and protein expression of myosin heavy chain in Nellore cattle comparing growth or meat tenderness traits. ANIMAL BIOTECHNOLOGY, v. 32, n. 3, p. 300-309, JUN 1 2021. (15/13021-1, 18/00981-5)

ENRIQUEZ-VALENCIA, CRUZ ELENA; PEREIRA, GUILHERME LUIS; MALHEIROS, JESSICA MORAES; DE VASCONCELOS SILVA, JOSINEUDSON AUGUSTO I. I.; ALBUQUERQUE, LUCIA GALVAO; DE OLIVEIRA, HENRIQUE NUNES; LOYOLA CHARDULO, LUIS ARTUR; CURI, ROGERIO ABDALLAH. Effect of the g.98535683A > G SNP in the CAST gene on meat traits of Nellore beef cattle (Bos indicus) and their crosses with Bos taurus. MEAT SCIENCE, v. 123, p. 64-66, JAN 2017. (09/16118-5, 15/13021-1)

MALHEIROS, JESSICA MORAES; ENRIQUEZ-VALENCIA, CRUZ ELENA; DE VASCONCELOS SILVA, JOSINEUDSON AUGUSTO I. I.; CURI, ROGERIO ABDALLAH; DE OLIVEIRA, HENRIQUE NUNES; DE ALBUQUERQUE, LUCIA GALVAO; LOYOLA CHARDULO, LUIS ARTUR. Carcass and meat quality of Nellore cattle (Bos taurus indicus) belonging to the breeding programs. LIVESTOCK SCIENCE, v. 242, DEC 2020. (15/13021-1, 09/16118-5)