The relative activity of calpastatin isoforms determine postmortem aging of beef from animals with great participation of Bos indicus

• Grant number: 14/24602-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2015
• End date: August 31, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Eduardo Francisquine Delgado
• Grantee: Eduardo Francisquine Delgado
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Angélica Simone Cravo Pereira ; Luciane Silva Martello ; Saulo da Luz e Silva

Abstract

The beef industry has socioeconomic importance to Brazil. Factors that favor the acceptability of the meat should be explored in order to stimulate consumption of a very important dietary ingredient, with special attention towards decreasing negative experiences. Tenderness is important as a quality criterion for this meat, so much that some consumers are willing to pay more for guaranteed tender meat. The predominant biological type in Brazil is the zebu cattle, and among these type Nellore is the most important representative. These animals are more aggressive and have tougher meat than taurine counterparts. The lower tenderness observed in zebu cattle seems to follow a similar pattern to that seen in more temperamental animals within a genotype. In both cases, the inhibitory activity of calpastatin on the proteolysis caused by ¼-calpain is involved and seems to be determinant of less tender meat. Several studies have presented evidences of increased activity of calpastatin by stimulation of ²-adrenergic receptors via the sympathetic nervous system, altering tenderness. There are also evidences of calpastatin isoforms responding differentially to that stimulation, and that they may have different inhibitory activity. For these reasons, this study aims to evaluate the relationship between the activities of calpastatin isoforms and tenderness of meat from animals of different biological groups or temperament in Bos indicus cattle, and thus to clarify the real cause of the difference in tenderness between Bos indicus and Bos taurus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, GIANCARLO DE MOURA; DA SILVA COUTINHO, MARCELO ARANDA; RAMOS, PATRICIA MALOSO; DE OLIVEIRA, GIULIANA MICAI; LONERGAN, STEVEN MICHAEL; DELGADO, EDUARDO FRANCISQUINE. Tough aged meat presents greater expression of calpastatin, which presents postmortem protein profile and tenderization related to Nellore steer temperament. MEAT SCIENCE, v. 156, p. 131-138, OCT 2019. (14/24602-2)

DA SILVA COUTINHO, MARCELO ARANDA; RAMOS, PATRICIA MALOSO; DA LUZ E SILVA, SAULO; MARTELLO, LUCIANE SILVA; CRAVO PEREIRA, ANGELICA SIMONE; DELGADO, EDUARDO FRANCISQUINE. Divergent temperaments are associated with beef tenderness and the inhibitory activity of calpastatin. MEAT SCIENCE, v. 134, p. 61-67, DEC 2017. (14/24602-2)