Assessment of the immature platelet fraction (IPF) and its association with clinical and laboratory manifestations in patients with Systemic Lupus Erythematosus systemic

• Grant number: 14/00735-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2014
• End date: July 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Simone Appenzeller
• Grantee: Amanda Meireles Silva
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease of the connective tissue of multisystemic involvement. Its etiology is unknown, mainly affecting women in the reproductive phase in the age group between 15-50 years of age. Characterized by diverse clinical manifestations, the disease is related to genetic, hormonal, environmental factors and some medicines.The disease is classified as chronic with periods of activity and remission. The criteria used for the diagnosis of SLE are standardized by the AmericanCollege of Rheumatology, being considered the presence of four or more symptoms such as skin lesions, muscoesqueléticas, hematologic, cardiac and neurological. It is mostly common in adulthood, butaround 20% of children and adolescents up to age 16 are affected. The prevalence is also higher in females, with the clinical manifestations similar to the adult except that renal impairment is more frequent among children, around 70-100%.This research aims to evaluate platelet parameters that reflect the degree of platelet activation and kinetic datrombocitopoiese, especially the immature platelet fraction (IPF), and its association with clinical and laboratory manifestations in patients with lupusErythematosus (SLE).This evaluation will be done by analyzing thewhole blood of patients followed in the outpatient clinic rheumatology clinics Hospitalde Unicamp under the age of 16. The analysis will be conducted in the XE-5000 SYSMEX device which allows the overall platelet count, immature platelet count (through the analysis of RNA by a platelet fluorescence response) and determination of IPF. In addition, levelsplasma thrombopoietin (TPO), a growth factor essential to the production of platelets is assessed by ELISA in order to further data on the kinetics of thrombocytopoiesis obtain.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Amanda Meireles. Evaluation of immature platelet fraction (IPF) and your association with clinical manifestations and laboratory in patients with systemic lupus erythematosus. 2016. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.