Evaluation of immature platelet fraction (IPF) and your association with clinical manifestations and laboratory in patients with systemic lupus erythematosus

Author(s)

Amanda Meireles Silva

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas
• Defense date: 2016-08-18

Examining board members

Simone Appenzeller; Alexandre Wagner Silva de Souza; Fernanda Loureiro de Andrade Orsi

• Advisor: Erich Vinicius De Paula; Simone Appenzeller

Abstract

ABSTRACT Introduction: The IPF is hemostatically more active and is considered an important mediator in the pathophysiology of autoimmune processes. Several lines of evidence indicate their participation in the pathophysiology of systemic lupus erythematosus (SLE) such as high levels of thromboxane, P-selectin, and microparticles of platelet origin. Objective:Determine the IPF and the mean platelet volume (MPV) in juvenile and adult SLE and possible associations with clinical and laboratory manifestations Methods: Consecutive patients followed were selected in the rheumatology clinic of the Hospital de Clinicas / Unicamp. The IPF parameter was obtained by the platelet count with fluorescent staining and reading in the reticulocyte channel of the automated hematology counters (Sysmex XE5000). VPM is obtained in the same equipment, the evaluation of the mean platelet volume by impedance method. Disease activity was assessed by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and cumulative damage by the Systemic Lupus International Collaborating Clinics / American College of Rheumatology Damage Index (SLICC / ACR DI). Statistical analyzes were performed using the Spearman correlation test p <0.05 was considered statistically significant. Results: We included 84 patients with JSLE (74 women), mean age 19.15 ± 4.45. The patient¿s disease duration was 6.40 ± 4.31 years. And 148 adult patients with SLE (138 women), mean age 40.67 ± 15.50. The patient¿s disease duration was 13.3 ± 7.12 years. The average IPF juvenile SLE was 3.33 ± 2.76 and 10.51 ± 0.91 in the VPM. No correlation was observed between IPF and disease activity (p = 0.7, r = 0.04) and cumulative damage (p = 0.64, r = 0, 003) MV with disease activity (p = 0 94; r = 0.008) and cumulative damage (p = 0.78, r = 0, 006). The average adult IPF SLE was 3.40 ± 2.43 and 10.59 ± 0.92 in the VPM. No correlation was observed between the IPF and disease activity (p = 0.970 r = - 8 0.003) and cumulative damage (p = 0.856; r = 0.04), VPM with disease activity (p = 0.339; r = - 0.079) and cumulative damage (p = 0.734, r = 0.03). Conclusion: The fraction of immature platelets (IPF) and mean platelet volume (MPV) in patients with SLE and JSLE were within normal limits. There was no association between IPF and clinical and laboratory manifestations in patients with JSLE. But longitudinal studies are needed to determine its role in the pathophysiology of SLE. Keyword: lupus, platelets, Hematologic counters (AU)

• FAPESP's process: 14/00735-3 - Assessment of the immature platelet fraction (IPF) and its association with clinical and laboratory manifestations in patients with Systemic Lupus Erythematosus systemic
• Grantee: Amanda Meireles Silva
• Support Opportunities: Scholarships in Brazil - Master