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Probiotic action of Lactobacillus spp. on Candida albicans infection: study in invertebrate model hosts

Grant number: 14/12458-4
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): March 01, 2015
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Juliana Campos Junqueira
Grantee:Rodnei Dennis Rossoni
Supervisor: Eleftherios Mylonakis
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Research place: Brown University, United States  
Associated to the scholarship:13/25181-8 - Isolation and characterization of Lactobacillus spp. from oral cavity and their probiotic action in Candida albicans: biofilm formation, infection in invertebrate models and EFG1, HWP1 and ALS1 gene expression, BP.DR


In vivo studies are fundamental to the study of microbial interactions of pathogenic microorganisms, and discovery of new antimicrobial agents. An animal model should reproduce the corresponding pathogenesis of infection in humans, including colonization and invasion from a specific route of entry and interaction with the host immune system. The aim of this study is to evaluate in vivo probiotic action between different Lactobacillus species in Candida albicans through induction of experimental candidiasis in invertebrate model of Galleria mellonella and Caenorhabditis elegans. Ten clinical strains of Lactobacillus from the oral cavity of individuals without caries will be studied. The strains of Lactobacillus will be inoculated with C. albicans in experimental models of infection hosts (Galleria mellonella and Caenorhabditis elegans). In G. mellonella model, experimental infection will be assessed by survival curves, quantification of fungal load in the hemolymph, hemocyte density and quantification of melanization. For the C. elegans model, the nematodes will be co-infected with C. albicans and Lactobacillus. After that, the infection will be evaluated by survival curves, count of CFU/mL in the intestinal tract of nematodes and study of filamentation of C. albicans. The results will be analyzed for selecting the best statistical test for each analysis of this study, considering a significance level of 5% test. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE BARROS, PATRICIA PIMENTEL; SCORZONI, LILIANA; RIBEIRO, FELIPE DE CAMARGO; DE OLIVEIRA FUGISAKI, LUCIANA RUANO; FUCHS, BETH BURGWYN; MYLONAKIS, ELEFTHERIOS; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS; ROSSONI, RODNEI DENNIS. Lactobacillus paracasei 28.4 reduces in vitro hyphae formation of Candida albicans and prevents the filamentation in an experimental model of Caenorhabditis elegans. Microbial Pathogenesis, v. 117, p. 80-87, . (15/09770-9, 13/25181-8, 14/12458-4)
ROSSONI, RODNEI DENNIS; FUCHS, BETH BURGWYN; DE BARROS, PATRICIA PIMENTEL; VELLOSO, MARISOL DOS SANTOS; CARDOSO JORGE, ANTONIO OLAVO; JUNQUEIRA, JULIANA CAMPOS; MYLONAKIS, ELEFTHERIOS. Lactobacillus paracasei modulates the immune system of Galleria mellonella and protects against Candida albicans infection. PLoS One, v. 12, n. 3, . (14/12458-4, 13/25181-8, 15/09770-9)

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