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Assessing of the Akt phosphorylation status in muscle tissue of adult rats, offspring of rats with periodontal disease

Grant number: 14/09555-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2014
Effective date (End): August 31, 2016
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal researcher:Doris Hissako Sumida
Grantee:Thaís Verônica Saori Tsosura
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The fetal environment has been suggested as a possible causal factor for diabetes mellitus. The phenomenon known as programming suggests that stimuli or aggression during intrauterine life may result in changes in physiology and metabolism of offspring, increasing the risk of diseases in adulthood. Studies have demonstrated that periodontal disease (PD) and adipose tissue augmentation elevate the level of plasmatic cytokines. And cytokines, such as TNF-alpha, have been associated with insulin resistance and reduced expression of GLUT4. The objective of this study is to evaluate the Akt serine phosphorylation status in gastrocnemius skeletal muscle of adult rats, offspring of rats with periodontal disease. For this purpose, male and female Wistar rats (2 months old) will be used. The rats will be divided into two groups: 1) with periodontal disease (PED), in which the disease is induced by ligation with silk thread around the 1st molar, 2) control rats (CN). Seven days after ligature placement, the rats of both groups will be placed for mating, verifying daily by vaginal smear, the day of copulation. Pregnant rats will be separated into individual boxes. When male offspring of these rats completed 75 days, will be performing the experiment: evaluation of the Akt phosphorylation status in gastrocnemius skeletal muscle by the method of "Western Blotting".