Development of a double loading system for meglumine antimoniate and its in vitro evaluation in macrophages infected with L. infantum

• Grant number: 14/12443-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2014
• End date: June 30, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Eneida de Paula
• Grantee: Vicky Vanessa Ortega Gaona
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Leishmaniosis refers to a complex of infectious illnesses caused by intramacrophage Leishmania that can be fatal if not properly treated. Visceral leishmaniosis is a neglected disease, related to poverty and considered by the World Health Organization as the sixth more relevant endemic disease in the world. In Brazil it is a zoonosis that has dogs as the main urban reservatory of the parasite, and its control includes euthanasia of serum positive animals. Just a few medicines are available for the treatment of Canine Visceral Leishmaniosis (CVL), and they present limitations related to toxicity, efficacy, price and therapeutic schemes. In this project we intend to develop and to characterize a new drug delivery system for Meglumine Antimoniate (AME) in double-loaded liposomes, i.e., that contains AME complexed in Hydropropyl-Beta-Cyclodextrin for the in vitro treatment of CVL. We also intend to evaluate the mechanisms involved in the internalization, intracellular traffic and co-location of liposomes and parasites inside DH82 canine macrophage cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORTEGA, VANESSA; GIORGIO, SELMA; DE PAULA, ENEIDA. Liposomal formulations in the pharmacological treatment of leishmaniasis: a review. Journal of Liposome Research, v. 27, n. 3, p. 234-248, 2017. (14/12443-7, 13/01536-1)

ORTEGA, VANESSA; RADAIC, ALLAN; DE JESUS, MARCELO BISPO; DE PAULA, ENEIDA; GIORGIO, SELMA. Improved efficacy of meglumine antimoniate incorporated in anionic liposomes against Leishmania infantum infecting canine macrophages. Journal of Pharmacy and Pharmacology, v. 74, n. 6, p. 9-pg., 2021-07-21. (14/12443-7, 15/23767-0, 13/01536-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GAONA, Vicky Vanessa Ortega. Development of sustained release system of meglumine antimoniate in liposomes and evaluation of its activity in DH82 macrophages infected with Leishmania infantum. 2016. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.