Immunomodulatory mechanisms of mesenchymal stem cells in glial reactivity and correlation with nervous system regenerative capacity after lumbar root axotomy

• Grant number: 13/16134-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2014
• End date: January 31, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Alexandre Leite Rodrigues de Oliveira
• Grantee: Luciana Politti Cartarozzi
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 15/08297-8 - Analysis of MHC class I-dependent reactivity of glial cell after nervous system lesions by in vivo two-photon imaging, BE.EP.DR

Abstract

The interaction between motoneurons and the circunjacent microenvironment has a crucial function for survival and regulation of functional state and synaptic connectivity. After trauma, indicative changes of dysfunction and degeneration initiate proximal and distal to lesion, however, the regenerative capacity of nervous system result in compensatory structural and functional remodeling of the circuitry. There are evidences that neurons and glial cells express high number of molecules that originally were taken as specific of immune system and that these molecules, like MHC class I, act mediating the communication between neurons and glial cells both in normal and pathological conditions. Aiming at alternatives for positively influence the nervous system regenerative capacity the mesenchymal stem cells are, nowadays, a viable option, because they are easy to obtain and expand, they have trophic properties and secrete bioactive molecules that act in the regenerative process. Among these molecules are neurotrophic factors, like BDNF, IL 4 and IFN´ that can modulate the immune response in the central nervous system after lesion. In this context, this work will evaluate in vitro the modulation by mesenchymal stem cells on glial cells and also the nervous system regenerative capacity after lumbar root axotomy and MSC administration, through morphologic and molecular approaches. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARTAROZZI, LUCIANA POLITTI; PEREZ, MATHEUS; KIRCHHOFF, FRANK; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE. Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice. CELLS, v. 8, n. 5, MAY 2019. (14/06892-3, 13/16134-6)

CARTAROZZI, LUCIANA POLITTI; RIEDER, PHILLIP; BAI, XIANSHU; SCHELLER, ANJA; RODRIGUES DE OLIVEIRA, ALEXANDRE LEITE; KIRCHHOFF, FRANK. In vivo two-photon imaging of motoneurons and adjacent glia in the ventral spinal cord. JOURNAL OF NEUROSCIENCE METHODS, v. 299, p. 8-15, APR 1 2018. (15/08297-8, 13/16134-6, 14/06892-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARTAROZZI, Luciana Politti. Estudo dos mecanismos imunomoduladores exercidos pelas células tronco mesenquimais sobre a reatividade das células gliais e correlação com a capacidade regenerativa após axotomia de raízes lombares. 2018. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.