Effect of the dose of FSH in gonadotrophic superstimulation on superovulatory response and in vivo production of embryos in Santa Inês ewes

• Grant number: 14/04614-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2014
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Emilia Franco Oliveira
• Grantee: Giovanna Serpa Maciel Feliciano
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The objective of this study is to evaluate the effect of the dose of exogenous FSH in gonadotrophic treatment on the superovulatory response and in vivo production of embryos in sheep. Twenty four Santa Inês ewes will submit to estrus synchronization with an intravaginal device of progesterone (CIDR ®) that will be inserted on day 0 and will remain till the day 8. Associated with the start of the protocol and removal of intravaginal device will be administered 37.5 ¼g of an analogue of PGF2± (Prolise ®). The gonadotrophic treatment will start 48 hours before the removal of progesterone (D6) while females are randomly divided into three groups according to the total dose of exogenous porcine FSH: G200 (control, n = 8) - 200 mg of FSH; G133 (n = 8) - 133 mg of FSH, and G100 (n = 8) - 100 mg of FSH. The total doses will be administered in eight applications at 12 hour intervals (20, 20, 15, 15, 10, 10, 5 and 5% FSH). In D6 (1st application of FSH) the females will also receive 300 IU of eCG. Conventional ultrasonographic evaluations (B-mode) and Doppler (two dimensional and three dimensional) will be performed in order to accompany the growth of the ovulatory follicular wave, vascularization of the follicular wall (vascular volume and characteristics of vessels) and the time of ovulation. Six days after natural matings, the embryos will be collected and evaluated morphologically and regarding the percentage of apoptotic cells. Statistical analyzes will be performed by SAS software version 9.2 and the level of significance is P <0.05. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACIEL, GIOVANNA SERPA; KAKO RODRIGUEZ, MARIANA GARCIA; CORREIA SANTOS, VICTOR JOSE; RAMIREZ USCATEGUI, RICARDO ANDRES; NOCITI, RICARDO PERECIN; MARONEZI, MARJURY CRISTINA; OLIVEIRA, CLARA SLADE; ROSSI FELICIANO, MARCUS ANTONIO; RUSSIANO VICENTE, WILTER RICARDO; DA FONSECA, JEFERSON FERREIRA; et al. Follicular dynamics and in vivo embryo production in Santa Ines ewes treated with smaller doses of pFSH. Animal Reproduction Science, v. 209, OCT 2019. (14/04614-6)

OLIVEIRA, M. E. F.; RIBEIRO, I. F.; RODRIGUEZ, M. G. K.; MACIEL, G. S.; FONSECA, J. F.; BRANDAO, F. Z.; BARTLEWSKI, P. M.. Assessing the usefulness of B-mode and colour Doppler sonography, and measurements of circulating progesterone concentrations for determining ovarian responses in superovulated ewes. REPRODUCTION IN DOMESTIC ANIMALS, v. 53, n. 3, p. 742-750, JUN 2018. (14/04614-6, 15/13350-5)

MACIEL, GIOVANNA SERPA; KAKO RODRIGUEZ, MARIANA GARCIA; CORREIA SANTOS, VICTOR JOSE; RAMIREZ USCATEGUI, RICARDO ANDRES; NOCITI, RICARDO PERECIN; MARONEZI, MARJURY CRISTINA; OLIVEIRA, CLARA SLADE; ROSSI FELICIANO, MARCUS ANTONIO; RUSSIANO VICENTE, WILTER RICARDO; DA FONSECA, JEFERSON FERREIRA; et al. Follicular dynamics and invivo embryo production in Santa Ines ewes treated with smaller doses of pFSH. Animal Reproduction Science, v. 209, p. 7-pg., 2019-10-01. (14/04614-6)

MACIEL, G. S.; RODRIGUEZ, M. G. K.; OLIVEIRA, C. S.; VRISMAN, D. P.; WENDLER DA ROCHA, R.; PEREIRA DA CAMARA BARROS, F. F.; TEIXEIRA, P. M.; USCATEGUI, R. A. R.; FELICIANO, M. A. R.; VICENTE, W. R. R.; et al. Percentage of apoptotic embryonic cells under exogenous porcine FSH dose effect in superovulation protocol of ewes. REPRODUCTION IN DOMESTIC ANIMALS, v. 52, p. 2-pg., 2017-08-01. (14/04614-6)

MACIEL, G.; RODRIGUEZ, M.; MARONEZI, M.; CAMPOS, G.; SILVA, P.; TREVIZAN, J.; USCATEGUI, R.; FONSECA, J.; FELICIANO, M.; VICENTE, W.; et al. Effect of the FSH dose on superovulatory response in Santa Ines ewes. REPRODUCTION IN DOMESTIC ANIMALS, v. 51, p. 1-pg., 2016-10-01. (14/04614-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FELICIANO, Giovanna Serpa Maciel. Superovulatory response and in vivo production of embryos in Santa Inês ewes in function of FSH dosage. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.