A possible synergistic action of propolis components on human laryngeal epidermoid carcinoma (HEp-2) cells: mechanisms of resistance and cell death

• Grant number: 14/13245-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2014
• End date: July 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Ethnopharmacology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: José Maurício Sforcin
• Grantee: Lívia Matsumoto da Silva
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Recently, propolis produced by Apis mellifera has been widely studied, due to its biological activities, such as antibacterial, antiviral, antifungal, antiinflammatory, immunomodulatory, antitumoral, among others. Data from literature have shown the action of propolis extracts and its isolated components, but the synergism between its constituents has not been investigated yet. This project aims to understand the synergistic action of phenolic compounds on the inhibition of human laryngeal epidermoid carcinoma (HEp-2) cells. The action of 3 compounds found in propolis samples from our region (cinnamic, p-coumaric and caffeic acid) and their combination will be evaluated according to their concentration in propolis composition, trying to identify the synergistic action more closely to propolis activity. The inner and outer morphology of HEp-2 cells will be assessed by transmission microscopy after incubation with the compounds over time. The mechanisms of HEp-2 cells resistance to the variables will be evaluated by glicoprotein-P expression and immunofluorescence, and a possible apoptotic action by flow cytometry and anexin V. The ethnopharmacological approach with biological assays may provide a better comprehension of the mechanism of action of propolis components, and alternative treatments associated or not to those already used against tumor cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, LIVIA M.; FRION-HERRERA, YAHIMA; BARTOLOMEU, ARIANE R.; GORGULHO, CAROLINA MENDONCA; SFORCIN, JOSE M.. Mechanisms involved in the cytotoxic action of Brazilian propolis and caffeic acid against HEp-2 cells and modulation of P-glycoprotein activity. Journal of Pharmacy and Pharmacology, v. 69, n. 11, p. 1625-1633, NOV 2017. (14/13245-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Lívia Matsumoto da. Possible synergic action of propolis components against human laryngeal epidermoid carcinoma cells (HEp-2): resistance and cell death mechanisms. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.