Relationship between stress, female sex hormones and oral microbiota: effect on volatile sulfur compounds production and beta-defensin role

• Grant number: 14/11481-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2014
• End date: August 31, 2016
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Fernanda Klein Marcondes
• Grantee: Patricia Oliveira de Lima
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated research grant: 11/50419-2 - Oral health and behavior: interdisciplinary perspectives and emotional aspects in the health-disease process, AP.TEM

Abstract

Halitosis is an unpleasant change of breath due to volatile sulfur compounds (VSC) production originated from bacterial metabolism on the amino acid degradation. Menstrual cycle and pre-menstrual syndrome can change oral homeostasis and influence on VSC production. Although over 90% of bad breath cases are due to oral bacteria activities, anxiety and stress have been suggested as possible etiologic agents that cause halitosis. In previous study, we observed that stress reduced beta-defensin (BD) concentration, an antimicrobial peptide that plays an important role in innate defense response. Therefore, the aim of the present study is to determine the oral microbiota profile in oral healthy patients that presented some level of stress; to evaluate the influence of beta-defensin (BD) and female sex hormones estradiol (E) and progesterone (P) on bacterial growth and production of VSC from microorganisms determined in the first part of the present study. Saliva samples of stressed and non-stressed undergraduate students from Dentistry course (samples previously collected in a prior study) will be evaluated regarding six VSC - bacterial species producing, by PCR-real time. Furthermore, two of the bacterial species, which present higher amounts in saliva samples, will be selected for the study of the treatment effects with BD, E and P on growth and viability on planktonic culture. In addition, gas chromatography (Oral Chroma®) will be performed to measure the VSC released direct from culture tubes, to evaluate the effect of BD, E and P on VSC production by these microorganisms.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA, PATRICIA OLIVEIRA; NANI, BRUNO DIAS; ROLIM, GUSTAVO SATOLLO; GROPPO, FRANCISCO CARLOS; FRANZ-MONTAN, MICHELLE; ALVES DE MORAES, ANTONIO BENTO; COGO-MULLER, KARINA; MARCONDES, FERNANDA KLEIN. Effects of academic stress on the levels of oral volatile sulfur compounds, halitosis-related bacteria and stress biomarkers of healthy female undergraduate students. JOURNAL OF BREATH RESEARCH, v. 14, n. 3, JUL 2020. (08/01641-1, 14/11481-2, 11/50419-2, 10/01642-8)

DE LIMA, PATRICIA OLIVEIRA; NANI, BRUNO DIAS; ALMEIDA, BARBARA; MARCONDES, FERNANDA KLEIN; GROPPO, FRANCISCO CARLOS; ALVES DE MORAES, ANTONIO BENTO; FRANZ-MONTAN, MICHELLE; COGO-MUELLER, KARINA. Stress-related salivary proteins affect the production of volatile sulfur compounds by oral bacteria. ORAL DISEASES, v. 24, n. 7, p. 1358-1366, OCT 2018. (13/26691-0, 11/50419-2, 14/11481-2)