Importance of the interaction among potato, tomato and sweet pepper in the epidemiology of tomato chlorosis virus

• Grant number: 14/15114-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2014
• End date: May 15, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Jorge Alberto Marques Rezende
• Grantee: Tatiana Mituti
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated research grant: 12/51771-4 - Begomovirus and Crinivirus in Solanaceae: molecular epidemiology and management strategies, AP.TEM

Abstract

Three solanaceous are considered of great economic importance: potato (Solanum tuberosum), tomato (Solanum lycopersicum) and sweet pepper (Capsicum annuum). Tomato chlorosis virus - ToCV (Genus Crinivirus) has recently been identified infecting these three species in producing areas in Brazil. There is few information on the epidemiology of ToCV involving these solanaceous, which are often grown near to each other. Therefore, this work aims to study the interaction among potato, tomato and sweet pepper in the epidemiology of ToCV. Furthermore, studies will be carried out to optimize the molecular method for detection of this virus in potato. Potato, tomato and sweet pepper plants infected with ToCV will be used as sources of inoculum for acquisition of the virus by the whitefly Bemisia tabaci biotype B and subsequent transmission to the same species, in tests with and without choice option. For the optimization of RT-PCR for detection of ToCV in potato, total RNA extracted from different tissues (young, intermediate, old leaves and tubers) will be analyzed separately. Different methods of total RNA extraction for RT-PCR will also be analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MITUTI, T.; MOLINA, J. P. EDWARDS; REZENDE, J. A. M.. Bioassays on the role of tomato, potato and sweet pepper as sources of Tomato chlorosis virus transmitted by Bemisia tabaci MEAM1. European Journal of Plant Pathology, v. 152, n. 3, p. 613-619, NOV 2018. (14/15114-4, 12/51771-4)