Effect of Calloselasma rhodostoma and Bothrops moojeni LAAO on apoptotic machinery of JAK2V617F positive strains of myeloproliferative neoplasms

• Grant number: 14/19127-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2014
• Effective date (End): August 31, 2015
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Fabíola Attié de Castro
• Grantee: Thaís Fontanezi Maciel
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis, AP.TEM

Abstract

The Myeloproliferative neoplasms (MPN) are diseases characterized by increased proliferation and resistance to apoptosis of mature myeloid lineage cells. The cell lines of Myeloproliferative Neoplasms investigated in this study will be SET-2 and HEL 92.1.7 originated from Polycythemia vera (PV) and essential thrombocythemia (TE) patients and positive for Janus kinase 2 (JAK2) mutation. This mutation results in an exacerbation of JAK2 kinase enzimatic activity generating changes in cellular signaling associated with proliferation and cell death. Despite numerous recent knowledge about the pathogenesis of these diseases, so far not been described effective therapy of patients. In this context, new treatments involving the modulation of apoptotic machinery of cells has been studied, such as snakes toxins. This study will determine the potential effects of L-amino acid oxidase Rhodostoma calloselasma and Bothrops moojeni on the apoptosis of SET-2 lineages and HEL 92.1.7 obtained from patients with Myeloproliferative Neoplasms. The data obtained from the development of this study may contribute to the description of new drugs for MPN patients treatment.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SARTIM, MARCO A.; PINHEIRO, MATHEUS P.; DE PADUA, RICARDO A. P.; SAMPAIO, SUELY V.; CRISTINA NONATO, M.. Structural and binding studies of a C-type galactose-binding lectin from Bothrops jararacussu snake venom. Toxicon, v. 126, p. 59-69, FEB 2017. (11/23236-4, 14/19127-3, 14/11266-4)

CRISTIANE TAVARES; THAÍS MACIEL; SANDRA BURIN; LUCIANA AMBRÓSIO; SANDRO GHISLA; SUELY SAMPAIO; FABÍOLA CASTRO. l-Amino acid oxidase isolated from Calloselasma rhodostoma snake venom induces cytotoxicity and apoptosis in JAK2V617F-positive cell lines. Revista Brasileira de Hematologia e Hemoterapia, v. 38, n. 2, p. 128-134, 2016-06-00. (11/23236-4, 14/19127-3)