The Myeloproliferative neoplasms (MPN) are diseases characterized by increased proliferation and resistance to apoptosis of mature myeloid lineage cells. The cell lines of Myeloproliferative Neoplasms investigated in this study will be SET-2 and HEL 92.1.7 originated from Polycythemia vera (PV) and essential thrombocythemia (TE) patients and positive for Janus kinase 2 (JAK2) mutation. This mutation results in an exacerbation of JAK2 kinase enzimatic activity generating changes in cellular signaling associated with proliferation and cell death. Despite numerous recent knowledge about the pathogenesis of these diseases, so far not been described effective therapy of patients. In this context, new treatments involving the modulation of apoptotic machinery of cells has been studied, such as snakes toxins. This study will determine the potential effects of L-amino acid oxidase Rhodostoma calloselasma and Bothrops moojeni on the apoptosis of SET-2 lineages and HEL 92.1.7 obtained from patients with Myeloproliferative Neoplasms. The data obtained from the development of this study may contribute to the description of new drugs for MPN patients treatment.
News published in Agência FAPESP Newsletter about the scholarship: