Chemokine responses in brain of deer mouse (Peromyscus maniculatus) experimentally infected with vesicular stomatitis virus

• Grant number: 14/20631-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 01, 2015
• End date: November 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Paulo César Maiorka
• Grantee: Leonardo Pereira Mesquita
• Supervisor: Elizabeth W. Howerth
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Georgia, Athens (UGA), United States
• Associated to the scholarship: 13/00629-6 - Evaluation of Immune and Inflammatory Responses in Experimentally Induced Encephalitis in Mice by Bovine Herpesvirus 5 (BHV-5) and Vesicular Estomatitis Virus (VSV) Infection., BP.DR

Abstract

The vesicular stomatitis virus (VSV) is a well known virus that causes encephalitis in mice when inoculated intranasally. The deer mouse, a native New World rodent, has been used as a model for VSV infection. VSV neuropathogenesis in deer mouse is similar as those observed in others rodent species, and, when inoculated intranasally, the VSV can spread throughout the central nervous system (CNS) of the deer mouse through retrograde transneuronal transport and viral spread within the ventricular system. Chemokines are a family of small soluble proteins which are important for chemotaxis, maturation and activation of inflammatory cells to the site of infection, including the CNS. In VSV infection in mice, many chemokines are expressed in CNS and may contribute to development of encephalitis. Many cells in the CNS, including neurons, microglia and astrocytes can produce different types of chemokines such as CCL2 (MCP-1), CCL5 (RANTES) and CXCL-10 (IP-10) under neuroinflammatory disorders, including viral infections. Although it is known that many chemokines are expressed during VSV infection, little is known about the role of each resident cell of CNS regarding chemokine production in VSV encephalitis. Therefore, the aim of the present study is to evaluate the expression of the CCL2 (MCP-1), CCL5 (RANTES) and CXCL-10 (IP-10) chemokines through immunohistochemistry in the brain of deer mouse infected with VSV-NJ in different days postinoculation (PI). Additionally, the histologic lesions and virus antigen distribution will be correlated with pattern of chemokines immunoreactivity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MESQUITA, L. P.; BRUHN, F. R. P.; MAIORKA, P. C.; HOWERTH, E. W.. Expression Kinetics of RANTES and MCP-1 in the Brain of Deer Mice (Peromyscus maniculatus) Infected with Vesicular Stomatitis New Jersey Virus. Journal of Comparative Pathology, v. 155, n. 4, p. 326-338, NOV 2016. (14/20631-8)

MESQUITA, L. P.; DIAZ, M. H.; HOWERTH, E. W.; STALLKNECHT, D. E.; NOBLET, R.; GRAY, E. W.; MEAD, D. G.. Pathogenesis of Vesicular Stomatitis New Jersey Virus Infection in Deer Mice (Peromyscus maniculatus) Transmitted by Black Flies (Simulium vittatum). VETERINARY PATHOLOGY, v. 54, n. 1, p. 74-81, JAN 2017. (14/20631-8)