Involvement of microRNAs on the accelerated ageing process caused by obesity

• Grant number: 14/20380-5
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 01, 2015
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Silvana Auxiliadora Bordin da Silva
• Grantee: Daniella Esteves Duque Guimarães
• Supervisor: Susan Ozanne
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Cambridge, England
• Associated to the scholarship: 14/09118-7 - The involvement of microRNAs in the accelerated ageing process caused by obesity and the potential intervention by caloric restriction, fatty acid supplementation and exercise, BP.PD

Abstract

Several studies have demonstrated the deleterious effects of the maternal obesity on the metabolism of the offspring including accelerating the onset of conditions traditionally associated with ageing, as type 2 diabetes. On the last years, Ozanne´s group has found the participation of an accelerated celular senescence process on the offspring of obese mothers. More recently, skeletal muscle was also identified as a tissue that has been involved to the process of early ageing as consequence of the maternal obesity. The understanding of the ageing process on the effects of maternal obesity on the offspring would add a robust contribution to the field, once they become diabetics later in life. Moreover the involvement of microRNAs (miRNAs) on this context represents another relevant mechanism that could bring a deeper contribution to the understanding of the consequences of maternal obesity on the offspring. Ozanne´s group has also showed that a distinct modification in the profile of miRNAs could be associated to an exposure to an obesogenic environment in utero. Considering that miRNAs are potentially biomarkers for detection of diseases, the main goal of the current project is identify dysregulated miRNAs and their targets on the offspring of obese mothers that could be closely related to the development of diabetes. For the purpose of this project, RNA-seq, proteomics-SILAC and in silico analysis will be used to achieve the goal. Thus, this project would build up a valuable contribution of making the early identification of individuals at high risk of developing diabetes possible. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUQUE-GUIMARES, DANIELLA; OZANNE, SUSAN. Early nutrition and ageing: can we intervene?. BIOGERONTOLOGY, v. 18, n. 6, SI, p. 893-900, DEC 2017. (14/20380-5)

FERNANDEZ-TWINN, DENISE S.; GASCOIN, GERALDINE; MUSIAL, BARBARA; CARR, SARAH; DUQUE-GUIMARAES, DANIELLA; BLACKMORE, HEATHER L.; ALFARADHI, MARIA Z.; LOCHE, ELENA; SFERRUZZI-PERRI, AMANDA N.; FOWDEN, ABIGAIL L.; et al. Exercise rescues obese mothers' insulin sensitivity, placental hypoxia and male offspring insulin sensitivity. SCIENTIFIC REPORTS, v. 7, MAR 14 2017. (14/20380-5)

MUSIAL, BARBARA; FERNANDEZ-TWINN, DENISE S.; DUQUE-GUIMARAES, DANIELLA; CARR, SARAH K.; FOWDEN, ABIGAIL L.; OZANNE, SUSAN E.; SFERRUZZI-PERRI, AMANDA N.. Exercise alters the molecular pathways of insulin signaling and lipid handling in maternal tissues of obese pregnant mice. PHYSIOLOGICAL REPORTS, v. 7, n. 16, AUG 2019. (14/20380-5)

FARIA, JULIANA DE ALMEIDA; DUQUE-GUIMARAES, DANIELLA; CARPENTER, ASHA A. M.; LOCHE, ELENA; OZANNE, SUSAN E.. A post-weaning obesogenic diet exacerbates the detrimental effects of maternal obesity on offspring insulin signaling in adipose tissue. SCIENTIFIC REPORTS, v. 7, MAR 24 2017. (14/20380-5, 14/17012-4)

DE ALMEIDA-FARIA, JULIANA; DUQUE-GUIMARAES, DANIELLA E.; ONG, THOMAS P.; PANTALEAO, LUCAS C.; CARPENTER, ASHA A.; LOCHE, ELENA; KUSINSKI, LAURA C.; ASHMORE, THOMAS J.; ANTROBUS, ROBIN; BUSHELL, MARTIN; et al. Maternal obesity during pregnancy leads to adipose tissue ER stress in mice via miR-126-mediated reduction in Lunapark. Diabetologia, v. 64, n. 4, JAN 2021. (14/17012-4, 17/03525-8, 14/20380-5, 13/07914-8)

DUQUE-GUIMARES, DANIELLA; OZANNE, SUSAN. Early nutrition and ageing: can we intervene?. BIOGERONTOLOGY, v. 18, n. 6, p. 8-pg., 2017-12-01. (14/20380-5)