Fluorescence spectroscopy for diagnosis of clinically similar skin lesions

• Grant number: 14/16154-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2014
• End date: July 31, 2016
• Field of knowledge: Biological Sciences - Biophysics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Cristina Kurachi
• Grantee: Marcelo Saito Nogueira
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

The fluorescence spectra and fluorescence lifetime analysis in biological tissues has been presented as a technique of great potential for tissue characterization for diagnostic purposes. The main fluorophores are NADH (Nicotinamide Adenine Dinucleotide) and FAD (Flavin Adenine Dinucleotide), biomolecules involved in cellular respiration that may provide information on the metabolism of the cell. One of the main advantages of optical fluorescence techniques for diagnosis is the possibility of evaluating the tissue metabolism in situ, without removal and processing of the biological sample. Skin lesions may be clinically classified into two major groups: pigmented and non-pigmented. In each group, there are benign lesions may be confused with clinically malignant lesions. The patient prognosis and the treatment modality depend on the histological type and the evolution of the lesion. Ancillary diagnostic techniques are very important to improve the planning and the success of treatment. The clinical diagnosis becomes more complex when lesions are clinically similar. The main objectives of this study are to obtain a database to characterize the fluorescence of NADH and FAD for different skin lesions and to evaluate the diagnostic resolution of fluorescence spectroscopy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA, MARCELO SAITO; COSCI, ALESSANDRO; TEIXEIRA ROSA, RAMON GABRIEL; SALVIO, ANA GABRIELA; PRATAVIEIRA, SEBASTIAO; KURACHI, CRISTINA. Portable fluorescence lifetime spectroscopy system for in-situ interrogation of biological tissues. Journal of Biomedical Optics, v. 22, n. 12, DEC 2017. (13/07276-1, 14/16154-0, 14/50857-8)

CARVALHO, LUIS FELIPE C. S.; NOGUEIRA, MARCELO SAITO; NETO, LAZARO P. M.; BHATTACHARJEE, TANMOY T.; MARTIN, AIRTON A.. Raman spectral post-processing for oral tissue discrimination - a step for an automatized diagnostic system. BIOMEDICAL OPTICS EXPRESS, v. 8, n. 11, p. 5218-5227, NOV 1 2017. (14/16154-0, 14/05978-1)

COSCI, ALESSANDRO; NOGUEIRA, MARCELO SAITO; PRATAVIEIRA, SEBASTIAO; TAKAHAMA, JR., ADEMAR; AZEVEDO, REBECA DE SOUZA; KURACHI, CRISTINA. Time-resolved fluorescence spectroscopy for clinical diagnosis of actinic cheilitis. BIOMEDICAL OPTICS EXPRESS, v. 7, n. 10, p. 4210-4219, OCT 1 2016. (13/07276-1, 14/16154-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOGUEIRA, Marcelo Saito. Fluorescence lifetime spectroscopy for diagnosis of clinically similar skin lesions. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.