Comparative study of the immunophenotypic profile, differentiation potential, capacity of production of cytocines and cryopreservation of endometrial stromal cells of cattle during the oestrous cycle

• Grant number: 14/20447-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2014
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Eunice Oba
• Grantee: Carolina Nogueira de Moraes Maia
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 15/18964-1 - Research training in characterization, cryopreservation, sorting, homing and gene therapy using MSCs in experimental models: evaluation of cytokine therapy using TRAIL associated to MSCs for the treatment of glioblastoma on animal model, BE.EP.DR

Abstract

Scientific discoveries have demonstrated the differentiation potential of stem cells from mesenchymal origin and its therapeutic application in both complex illnesses or injuries difficult to repair as in gene therapy. The aim of this study is to isolate, cultivate and characterize progenitor stromal cells of endometrial tissue from cows in phases II and III of corpus luteum formation. Additionally, the quantification of cytokine production by stromal progenitor cells under stimulation of LPS will be held, as well as the evaluation of viability pre and post-cryopreservation with two mediums of cryopreservation. For this purpose, 12 samples of endometrial tissue obtained from a slaughterhouse will be used, with 6 animals in the luteal phase II (n = 6) and 6 animals in the luteal phase III (n = 6). After harvest, the material will be properly manipulated in the laboratory to LANCA for isolation, culture and expansion in vitro of stromal cells. Later, it will be done the immunophenotypically characterization of cultured cells and also the differentiation for the tri- lineages. Also, cells will be stimulated with LPS for evaluating the immunomodulatory profile. Finally, the cells will be cryopreserved in two mediums of different cryopreservation and assessed for viability after one month of freezing. This experiment will provide data retrieval harvesting, cultivation, characterization, immunomodulatory profile and viability after cryopreservation of endometrial stromal cells from the tissue of cattle in view of the scarcity of studies on this species. Additionally, the data can provide a better understanding for the study of the pathophysiology of uterine disease in cattle, as well as provide better knowledge of the therapeutic application of stromal progenitor cells against these diseases, and the possible creation of cell banks for use and future studies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA DE MORAES, CAROLINA; MAIA, LEANDRO; DE OLIVEIRA, ELIANDRE; DE PAULA FREITAS DELL'AQUA, CAMILA; CHAPWANYA, ASPINAS; DA CRUZ LANDIM-ALVARENGA, FERNANDO; OBA, EUNICE. Shotgun proteomic analysis of the secretome of bovine endometrial mesenchymal progenitor/stem cells challenged or not with bacterial lipopolysaccharide. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v. 187, p. 42-47, MAY 2017. (15/01057-1, 15/18964-1, 14/20447-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MAIA, Carolina Nogueira de Moraes. Comparative study of the immunophenotypic profile, differentiation potential, capacity of production of cytokines and cryopreservation of endometrial stromal cells of cattle during the oestrous cycle. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp).