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Assessment of the effect of the dental pigmentation intensity in the trans-enamel and trans-dentinal penetration of hydrogen peroxide

Grant number: 14/11748-9
Support Opportunities:Scholarships in Brazil - Master
Start date: December 01, 2014
End date: January 31, 2016
Field of knowledge:Health Sciences - Dentistry
Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:André Luiz Fraga Briso
Grantee:Janaina Cardoso Moreira
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The purpose of the present study is to evaluate the color alteration and hydrogen peroxide trans-enamel and trans-dentinal penetration in bovine teeth, pigmented using black tea in different intensities. Firstly, the teeth will be analyzed and selected according to L* value, from the CIE L*a*b* color system. Afterwards, the specimens (N=80) will be divided into 4 groups (n=20) according to the pigment solution used: G1 - immersed in distilled water, G2, G3 and G4 - immersed for 6 days in black tea in the following concentrations: 10, 100 and 1000 mL of distilled water in 1.6 g of black tea, respectively. Thus, the samples will be re-analyzed using a spectrophotometer device Easyshade (Vita-Zahnfabrik, Alemanha), resulting in a new selection of the specimen according to the pigmentation level (n=15). In order to quantify the peroxide penetration, specimens will be positioned in Artificial Pulp Chambers (APC) and submitted to the bleaching treatment using 38% hydrogen peroxide (Opalescence Xtra Boost - Ultradent Products Inc., South Jordan, UT, USA). An acetate buffer solution inside the pulp chambers will be collected and after the addition of the enzyme peroxidase it will be evaluated in the reflectance spectroscopy. The data will be submitted to the most appropriate statistical analysis at a 5% level of significance. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JANAINA CARDOSO MOREIRA; MARJORIE DE OLIVEIRA GALLINARI; VANESSA RAHAL; TICIANE CESTARI FAGUNDES; PAULO HENRIQUE DOS SANTOS; MARCIA REGINA DE MOURA; ANDRÉ LUIZ FRAGA BRISO. Effect of Dental Pigmentation Intensity on the Transenamel and Transdentinal Penetration of Hydrogen Peroxide. Brazilian Dental Journal, v. 27, n. 4, p. 399-403, . (15/01366-4, 14/11748-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MOREIRA, Janaina Cardoso. Effect of dental pigmentation intensity on the transenamel and transdentinal penetration of hydrogen peroxide. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.