Advanced search
Start date

Estrogenic and biochemical effects of N-(3,4-dichlorophenyl)-N, N-dimethylurea (diuron) and its metabolites, alone or in combination with alkylphenols in Nile Tilapia (Oreochromis niloticus)

Grant number: 14/18825-9
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2014
Effective date (End): February 28, 2017
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Eduardo Alves de Almeida
Grantee:Andréia Arantes Felício
Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil


The anthropogenic activity has increased alarmingly, due to development, and increasingly are released mixtures of different substances in the environment, increasing levels of environmental contamination. The development leads to the production of products such as fuels, and among them there is ethanol. With the increased production of ethanol in the culture of sugar cane, and consequently, the use of pesticides, one of which is diuron (N-(3,4-dichlorophenyl)-N, Ndimetilureia), widely used for combating weeds in various crops, including sugar cane. Diuron can be degraded in other products, mostly by microbial means (biodegradable) and they 3,4-dichloroaniline (3,4-DCA), 3 - (3,4-dichlorophenyl)-3-methylurea (DCPMU) and 3,4-diclorofenilureia (DCPU). Some substances such as alquilfenólicos polyethoxylates (APE), nonylphenols (NPE) and octylphenol ethoxylate (OPE) are used in combination with diuron to increase its solubility. These substances can cause estrogenic effects in fish and can be detected by the concentration of plasma steroid hormones (testosterone, 11-cetotestosterona and 17b-estradiol). In addition to estrogen effects, other effects can be detected by analysis of biomarkers from contamination. Therefore, the project aims to study the deleterious effects of diuron and its main metabolites of biodegradation, alone and in combination with alkylphenols (AP) at different concentrations in Oreochromis niloticus (Nile tilapia) exposed for seven days. To evaluate the effects will be analyzed the following parameters: EROD, PROD, BROD, glutathione S-transferase, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-6-phosphate, lipid peroxidation and oxidative DNA damage, comet assay , micronucleus test, and the levels of vitelogenia and of the hormones 11-cetotestosterona, 17b - estradiol and testosterone levels in plasma and finally enzyme activity of steroid hormone metabolism 17b-estradiol dehydrogenase and aromatase. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FELICIO, ANDREIA A.; CRAGO, JORDAN; MARYOUNG, LINDLEY A.; ALMEIDA, EDUARDO A.; SCHLENK, DANIEL. Effects of alkylphenols on the biotransformation of diuron and enzymes involved in the synthesis and clearance of sex steroids in juvenile male tilapia (Oreochromus mossambica). AQUATIC TOXICOLOGY, v. 180, p. 345-352, . (11/52061-8, 14/18825-9)
FREITAS, JULIANE SILBERSCHMIDT; FELICIO, ANDREIA ARANTES; TERESA, FABRICIO BARRETO; DE ALMEIDA, EDUARDO ALVES. Combined effects of temperature and clomazone (Gamit (R)) on oxidative stress responses and B-esterase activity of Physalaemus nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae) tadpoles. Chemosphere, v. 185, p. 548-562, . (13/16948-3, 14/18825-9)
FELICIO, ANDREIA ARANTES; FREITAS, JULIANE SILBERSCHMIDT; SCARIN, JESSICA BOLPETI; ONDEI, LUCIANA DE SOUZA; TERESA, FABRICIO BARRETO; SCHLENK, DANIEL; DE ALMEIDA, EDUARDO ALVES. Isolated and mixed effects of diuron and its metabolites on biotransformation enzymes and oxidative stress response of Nile tilapia (Oreochromis niloticus). ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 149, p. 248-256, . (11/52061-8, 13/16948-3, 14/18825-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FELÍCIO, Andréia Arantes. Biochemical and estrogenic effects of N- (3,4-dichlorophenyl) -N, N-dimethylurea (diuron) and its metabolites, alone or in combination with alkylphenols in Nile tilapia (Oreochromis niloticus). 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

Please report errors in scientific publications list by writing to: