High scale genomic analysis applied to B chromosome biology

• Grant number: 14/16477-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2015
• End date: September 04, 2019
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: César Martins
• Grantee: Syed Farhan Ahmad
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 18/03877-4 - Exploration of next generation sequencing data to uncover the genomic contents and origin of B chromosomes., BE.EP.DR

Abstract

Approximately 15% of eukaryotes contain supernumerary B chromosomes. When present, B chromosomes frequently represent as much as 5% of the genome. Despite thousands of reports describing the distribution of supernumeraries in various taxa, a comprehensive theory for the origin, maintenance, and evolution of B chromosomes has not emerged. Here, we propose sequencing the complete genomes of different species (the plant Cestrum intermedium, the coleopteran beetle Dichotomius geminatus and fish species Astyanax mexicanus) with and without B chromosomes to identify DNA sequences on the B. B sequences will further be analyzed through quantitative polymerase chain reaction and in situ hybridization. Genome sequencing based on Illumina next generation sequencing, comparative analysis, gene annotation and mapping and identification of B chromosomes will be performed. The development of the present research proposal will allow the identification of genes present on B chromosomes of different species and their possible biological role. Furthermore, we will be able to test the hypothesis that cell cycle genes tend to accumulate independently on B chromosomes of different species. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AHMAD, SYED F.; JEHANGIR, MARYAM; CARDOSO, ADAUTO L.; WOLF, IVAN R.; MARGARIDO, VLADIMIR P.; CABRAL-DE-MELLO, DIOGO C.; O'NEILL, RACHEL; VALENTE, GUILHERME T.; MARTINS, CESAR. B chromosomes of multiple species have intense evolutionary dynamics and accumulated genes related to important biological processes. BMC Genomics, v. 21, n. 1, SEP 23 2020. (18/03877-4, 15/16661-1, 14/16477-3)

AHMAD, SYED FARHAN; MARTINS, CESAR. The Modern View of B Chromosomes Under the Impact of High Scale Omics Analyses. CELLS, v. 8, n. 2, FEB 2019. (15/16661-1, 14/16477-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AHMAD, Syed Farhan. High Scale Genomic Applied to B chromosome biology. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.