Study of anti-Mullerian hormone in mares: relationship with age and expression of ovarian receptors

• Grant number: 15/01113-9
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): April 15, 2015
• Effective date (End): July 14, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marco Antonio Alvarenga
• Grantee: Renata Cristina Uliani
• Supervisor abroad: Alan James Conley
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Local de pesquisa : University of California, Davis (UC Davis), United States
• Associated to the scholarship: 14/13191-1 - Study of anti-Mullerian hormone in mares: relationship with age and expression of ovarian receptors, BP.DR

Abstract

In humans it is known that serum anti-Müllerian hormone (AMH) decline with age and is strongly associated to the number of follicles present in the ovaries. Today, in human medicine, it is recognized that the measurement of AMH in serum is the most accurate measure of ovarian reserve has been used to predict both, fertility and success in assisted reproduction programs. Published works with AMH are recent in the literature, with promising results that justify new studies in order to apply it in clinical practice of equine reproduction, the way it has been doing with humans. Thus, the "Estágio de pesquisa no exterior (BEPE)" will be held in two stages. The first part will be held at "Gluck Equine Research Center" at the University of Kentucky in Lexington. At this stage, the student will train for performing the immunohistochemical technique for equine ovaries, in order to identify the production of the Anti-Mullerian Hormone in young and old mares' ovaries. This technique is well established in this lab. The second phase will be held in "Clinical Laboratory Endocrinoloy" at the University of California in Davis, under the supervision of Dr. Alan J. Conley. At this time, the student will perform, along with the laboratory staff, hormonal dosage for Anti-Mullerian of blood samples collected from 520 mares in Brazil. The student will learn to develop the ELISA technique, already commercially carried out by that laboratory. The two research centers to be visited work together and are the only ones to study Anti-Mullerian Hormone in mares in the world, being the only ones with published works about this topic. Certainly the stage will be enriching for both the project in question and for future studies, partnering with renowned researchers and bringing credibility to the work to be published as well as new technologies to be developed in our laboratory. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ULIANI, RENATA C.; CONLEY, ALAN J.; CORBIN, C. JO; FRISO, AIME M.; MACIEL, LUCIANA F. S.; ALVARENGA, MARCO A. Anti-Mullerian hormone and ovarian aging in mares. Journal of Endocrinology, v. 240, n. 2, p. 147-156, FEB 2019. Web of Science Citations: 2.