Physiological and molecular responses of three models of running training with equalized loads in Wistar rats

• Grant number: 14/19627-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): March 01, 2015
• Effective date (End): July 31, 2015
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Marcelo Papoti
• Grantee: Carlos Dellavechia de Carvalho
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Studies have shown improvement in oxidative activity and performance from different models of training. However, few studies analyzed the physiological responses from the manipulation of the variables volume and intensity with equalization of loads. The aim of this study is to verify the physiological and molecular responses of Wistar rats submitted to three acute training sessions, with equivalent loads. Seventy-eight rats will be subjected to an incremental test to determine the anaerobic threshold (AnT) and after 48 hours, 6 rats will be sacrificed for determination of baseline (GBase), while the others (n=72) rats will be divided randomly into 3 groups. The training sessions will be: GZ1 (n=24), continuous running in intensity below the AnT (volume=43min, intensity=70% AnT); GZ2 (n=24) one running will be held at AnT intensity (volume=31min, AnT intensity=100%) and GZ3 (n=24), efforts supra-AnT (volume=25min, intensity=120.4% AnT). Before and after acute sessions and after 6, 12 and 24 hours, 6 animals in each group will be sacrificed for quantification of muscle content (gastrocnemius and soleus) of glycogen, glucose transporter (GLUT-4), proliferator-activated receptor- preroxisome y coativator 1-± (PGC1-±), AMP-activated protein kinase (AMPK) and citrate synthase (CS) by means of immunoprecipitation and immunoblot techniques. For comparison between groups (GZ1, GZ2, GZ3) at different times after training the One-way analysis of variance test (ANOVA) and Tukey post-hoc test will be used, with p<0.05.