Identification of degradation products of cefepime hydrochloride by LC-HRMS

• Grant number: 15/01551-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 30, 2015
• End date: December 29, 2015
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Hérida Regina Nunes Salgado
• Grantee: Danilo Fernando Rodrigues
• Supervisor: Demian R. Ifa
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: York University, Canada
• Associated to the scholarship: 13/20017-5 - Chemical-pharmaceutical analysis of Cefepime hydrochloride in lyophilized powder for injectable solution, BP.DR

Abstract

The emergence of resistant microorganisms to a large number of clinically approved antimicrobials has been increasingly used, which limits the options for the treatment of bacterial infections. As a strategy, drugs with high antimicrobial activities are in evidence. Stands out a class of antimicrobial, the cephalosporins, having as fourth generation cefepime a semi-synthetic product which has activity against various Gram-positive bacteria (e.g. oxacillin resistant Staphylococcus aureus) and Gram-negative (e.g. Pseudomonas aeruginosa) aerobic. Researches involving the forced degradation of a drug are of paramount importance for the understanding of the chemical behavior of the molecule. This study identifies not only the possible degradation products, but also provides relevant details for the development of new formulas and their packaging. The work aims at achieving forced degradation studies including acidic conditions, basic, aqueous, photolysis and oxidative conducted with cefepime hydrochloride to identify the possible degradation products formed, by liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Thus, these cefepime hydrochloride stability studies will be essential to ensure the efficacy and safety of new products. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, DANILO F.; SALGADO, HERIDA R. N.. Development and Validation of an Leo-Friendly and Low-Cost Method for the Quantification of Cefepime Hydrochloride in Powder for Injectable Solution Using Infrared (IR) Spectroscopy. CURRENT ANALYTICAL CHEMISTRY, v. 16, n. 4, p. 456-464, 2020. (15/01551-6, 13/20017-5)