Nanostructures and iPSC derived cardiomyocytes approaches for cardiac protection and repair post-MI

• Grant number: 14/24577-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2015
• End date: December 31, 2018
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: José Eduardo Krieger
• Grantee: Rafael Dariolli
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 13/17368-0 - Cardiovascular genomics: mechanisms & novel therapeutics - CVGen mech2ther, AP.TEM
• Associated scholarship(s): 16/07541-5 - Action potential and Calcium transient phenotyping of cardiomyocytes derived from pluripotent stem cells: development of an electrophysiological platform CM-PSCs compatible to study disease and drug modeling and cardiac regeneration approaches, BE.EP.PD

Abstract

Induced pluripotent stem cells (iPSCs) hold great promise for cell therapy including cardiac protection and replacement of damaged cardiomyocytes after a myocardial infarction (MI). The main goal of this proposal is to test the hypothesis that lockyballs nanostructures increase the efficacy of stem cell transplantation to protect and regenerate the myocardium post-MI in humanized pigs. Lockyballs nanostructures will be used to increase the efficacy of cardiac cell retention to produce paracrine factors (adult stem cells) or to replace cardiomyocytes (iPSCs derived cardiomyocytes). In addition, sophisticated methodology will be used for phenotype quantification to better characterize electrophysiological properties of iPSCs derived cardiomyocytes. Taken together, the results of the present studies may contribute to 1. Identify the electrophysiological properties of iPSCs derived cardiomyocytes used to disease modeling or cell replacement approaches; 2. To increase the efficacy of adult stem cell transplantation to protect the myocardium post-MI; and 3. To establish strategies to replace cardiomyocytes in porcine model post-MI. This proposal is of great interest in the area to accelerate the development of novel alternative or complementary strategies to regenerate the myocardium post-MI.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MUNOZ, JUAN J. A. M.; DARIOLLI, RAFAEL; DA SILVA, CAIO MATEUS; NERI, ELIDA A.; VALADAO, IURI C.; TURACA, LAURO THIAGO; LIMA, VANESSA M.; PERES DE CARVALHO, MARIANA LOMBARDI; VELHO, MARILIZA R.; SOBIE, ERIC A.; et al. Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation. STEM CELL RESEARCH & THERAPY, v. 13, n. 1, p. 27-pg., 2022-09-02. (16/07541-5, 14/50889-7, 13/17368-0, 15/50216-5, 14/24577-8)

DA SILVA, AGATHA RIBEIRO; NERI, ELIDE A.; TURACA, LAURO THIAGO; DARIOLLI, RAFAEL; FONSECA-ALANIZ, MIRIAM H.; SANTOS-MIRANDA, ARTUR; ROMAN-CAMPOS, DANILO; VENTURINI, GABRIELA; KRIEGER, JOSE E.. NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro. SCIENTIFIC REPORTS, v. 10, n. 1, p. 18-pg., 2020-09-30. (19/21304-4, 14/24577-8, 18/22830-9, 15/50216-5, 17/05829-4, 13/17368-0, 14/09861-1)